1,3,7,9-四甲基尿酸抗抑郁作用的实验研究

目的探讨1,3,7,9-四甲基尿酸(theacrine,TC)的抗抑郁作用。方法采用悬尾实验、强迫游泳实验、自主活动实验、育亨宾(yohimbine)毒性实验、利血平(reserpine)实验以及5-羟色胺酸(5-HTP)诱导小鼠甩头行为等动物模型(各组剂量分别为3、10及30mg·kg-1,bid,连续7d灌胃给药)来评价TC的抗实验性抑郁效果及其可能的作用机制。结果与空白对照组相比,TC能明显缩短小鼠悬尾不动时间(P<0.01)及增加强迫游泳小鼠拨动转轮旋转次数(P<0.05),且对自主活动无影响。TC可一定程度上增强yohim-bine诱发实验动物死亡率,增强5-HTP导致的甩头行为(P<0.01)。此外,TC也具有明显地改善reserpine引起的小鼠体温下降(P<0.01),运动不能(P<0.05)和眼脸下垂(P<0.01)的作用。结论TC在多种抑郁模型上显示一定的药理活性,这些作用可能与影响单胺类神经递质有关。

Experimental study of theacrine on antidepressant effects

Aim To investigate the antidepressant effects of theacrine (TC).Methods The antidepressant effects of TC were examined by a series of experiments including tail suspension test,forced swimming test,ambulatory activity test,yohimbine induced toxicity test,reserpine test and 5-HTP induced head-twitching test(The doses of groups were 3,10,30 mg·kg-1,bid respectively for continued 7 days of intragastric administration).Results Compared with the control group,TC could effectively shorten the immobility time in the tail suspension test (P<0.05)and enhance the rotatory locomotor activities during forced swimming test (P<0.05),but didn't affect the ambulatory activity.Moreover,TC also enhanced the mortality induced by yohimbine,and increased the head-twitching number induced by 5-HTP (P<0.01).In addition,TC could markedly improve such symptoms as hypothermia (P<0.01),akinesia (P<0.05),and eye ptosis of mice (P<0.01) induced by reserpine.Conclusions TC has antidepressant effects in various depression models of mice,which may be contributed to its influence on monoamine neurotransmitter.