| 大鼠血红素加氧酶-1表达对呼吸机相关性肺损伤时SOD、MDA的影响 |
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| 引用本文： | 胡伟伟,赵文静.大鼠血红素加氧酶-1表达对呼吸机相关性肺损伤时SOD、MDA的影响[J].徐州医学院学报,2010,30(5):281-284. |
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| 作者姓名： | 胡伟伟 赵文静 |
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| 作者单位： | 江苏省麻醉与镇痛应用技术实验室,江苏,徐州,221002 |
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| 基金项目： | 江苏省科技厅社会发展项目，江苏省"六大人才高峰"第六批资助项目 |
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| 摘 要： | 目的以大鼠呼吸机相关性肺损伤（VILI）为模型,用血晶素（hemin）诱导大鼠血红素加氧酶-1（HO-1）表达,观察VILI时超氧化物歧化酶（SOD）和丙二醛（MDA）活性变化及HO-1对SOD和MDA的影响,探讨在VILI过程中的抗氧化应激保护作用及其机制。方法32只雄性SD大鼠随机分成4组（每组n=8）：对照组只做气管切开术,保留自主呼吸;模型组气管切开后行机械通气4 h;诱导剂组于模型制备前24 h腹腔注射血晶素40μmol/kg;抑制剂组于模型制备前24 h腹腔注射锌原卟啉（ZnPP）10μmol/kg。机械通气4 h后处死大鼠,收集肺组织和支气管肺泡灌洗液（BALF）标本,测定BALF中总蛋白含量,肺组织湿/干重比值（W/D）,肺组织LDH、SOD活性和MDA含量,检测肺组织HO-1蛋白表达,光镜下行肺组织病理学观察。结果与对照组相比,模型组大鼠肺组织病理损伤严重,肺W/D、BALF中总蛋白、LDH活性均明显增加,VILI模型复制成功。与模型组比较,诱导剂组肺组织HO-1表达增加,肺组织病理损伤明显减轻,SOD活性明显增加而MDA含量明显下降,用ZnPP抑制HO-1表达,此种保护作用消失。结论血晶素诱导大鼠HO-1表达可以增加SOD活性,降低MDA含量,减轻肺的氧化应激损伤,降低VILI的程度。
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| 关 键 词： | 呼吸机相关性肺损伤 血红素加氧酶-1 超氧化物歧化酶 丙二醛 |
The effect of HO-1 expression on SOD and MDA in ventilator-induced lung injury in rats |
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| HU Weiwei,ZHAO Wenjing.The effect of HO-1 expression on SOD and MDA in ventilator-induced lung injury in rats[J].Acta Academiae Medicinae Xuzhou,2010,30(5):281-284. |
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| Authors: | HU Weiwei ZHAO Wenjing |
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| Institution: | HU Weiwei,ZHAO Wenjing(Jiangsu Province Key Laboratory of Applied Technology for Anesthesia , Analgesia,Xuzhou,Jiangsu 221002,China) |
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| Abstract: | Objective To observe the variations in superoxide dismutase(SOD) activity and malondialdehyde(MDA) levels in ventilator-induced lung injury(VILI) and the effects of HO-1 expression on SOD and MDA with the establishment of rat model of VILI and the induction of heme oxygenase-1 expression by hemin,and to investigate the protective effect and the underlying mechanism of anti-oxidative stress in VILI.Methods 32 male Sprague-Dawley rats were randomized into 4 groups: control group(tracheotomy only,with spontane... |
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| Keywords: | ventilator-induced lung injury heme oxygenase-1 superoxide dismutase malondialdehyde |
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