| 蛋白质模板印迹法制备纳米“孔穴”结构特异性生物材料 |
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| 作者姓名： | Lu SL Cheng GX Cai ZJ Pang XS |
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| 作者单位： | 天津大学材料科学与工程学院,天津,300072 |
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| 基金项目： | 教育部优秀青年教师基金(2002123),国家自然科学基金(29906008)资助~~ |
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| 摘 要： | 以蛋白质为模板进行分子印迹得到的纳米“孔穴”结构生物材料,可以作为抗体、酶或其他天然生物结构的替代物及细胞支架材料,在生物技术和医学领域显示出广阔的应用前景。本文简要介绍了模板印迹的基本原理,分析了蛋白质模板印迹的特点,详细介绍了蛋白质包埋法、微球表面印迹法、平板表面印迹法和抗原表位法等蛋白质模板印迹方法,并对各种印迹方法进行了简要评述。
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| 关 键 词： | 模板印迹 分子印迹聚合物 蛋白质 纳米 孔穴 结构 生物材料 |
| 修稿时间： | 2002年6月25日 |
Preparation methods of nanocavity biomaterials with recognition specificity via template imprinting of proteins |
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| Lu SL,Cheng GX,Cai ZJ,Pang XS.Preparation methods of nanocavity biomaterials with recognition specificity via template imprinting of proteins[J].Acta Academiae Medicinae Sinicae,2003,25(5):640-644. |
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| Authors: | Lu Shu-lai Cheng Guo-xiang Cai Zhi-jiang Pang Xing-shou |
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| Institution: | School of Materials Science & Engineering, Tianjin University, Tianjin 300072, China. |
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| Abstract: | Nanocavity biomaterials with recognition specificity imprinted by using proteins as templates may successful serve as substitutes for antibodies,enzymes,and other native biological structures as well as cell bracket materials.It has numerous applications in biotechnology,medicine and so on.In this paper,the principle of template imprinting is introduced briefly,the specialty of template imprinting of proteins is analyzed,and the methods of template imprinting of proteins including protein entrapment,microbead surface imprinting,flat surface imprinting as well as the epitope are reviewed in details. |
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| Keywords: | template imprinting molecularly imprinted polymers protein nanocavity biomaterials |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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