miR-135a-5p抑制胆囊癌细胞增殖的研究

目的:探讨miR-135a-5p对胆囊癌的作用及其机制。方法:采用实时PCR检测miR-135a-5p和VLDLR在23对胆囊癌与癌旁组织的表达。采用miR-135a-5p模拟物转染胆囊癌细胞,通过细胞增殖(CCK-8)曲线、单克隆集落形成实验和细胞周期实验(流式细胞仪),检测miR-135a-5p对胆囊癌细胞增殖的影响。用Western印迹和Luciferase双荧光素酶报告系统验证和VLDLR受miR-135a-5p的调控。结果:在23对胆囊癌与癌旁组织中,癌组织的miR-135a-5p表达水平低于癌旁组织。体外实验证实miR-135a-5p通过G1期阻滞抑制胆囊癌细胞的增殖,且可通过作用于VLDLR mRNA降低其蛋白质水平。结论:miR-135a-5p抑制胆囊癌的增殖,可能是胆囊癌治疗靶点。

miR-135a-5p inhibits the proliferation of gallbladder cancer

Objective To investigate the role of miR-135a-Sp in gallbladder cancer and its potential mechanism. Methods The expression of miR-135a-Sp and very low density lipoprotein receptor （VLDLR） was analyzed by real-time PCR in 23 pairs of gallbladder cancer and paracaneerous tissues. For miR-135a-Sp mimic was transfected into gallbladder cancer cell line, and tests including CCK-8 assay and colony formation assay and cell cycle analysis （flow cytometry） were done. Luciferase reporter assays and Western blot were performed to study the potential target of miR-135a-Sp. Results miR-135a-Sp was downregulated in 23 pairs of gallbladder cancer compared with that of paracancerous tissues, miR-135a- 5p could obviously inhibit cancer cells proliferation in vitro through G1 arrest. VLDLR whose protein level and mRNA level could be down-regulated by miR-135a-Sp may be the potential functional target of miR-135a-Sp in gallbladder cancer. Conclusion The results suggested that miR-135a-Sp inhibits gallbladder cancer cell proliferation, indicating miR-135a may be a potential therapy target of gallbladder cancer.