M-CSF、IL-10对单核细胞IL-12、IL-18产生及HLA-DR和CD80表达的影响

目的 :探讨巨噬细胞集落刺激因子 (M CSF)和白细胞介素 10(IL 10 )对人外周血单核细胞分泌IL 12、IL 18及细胞表面HLA DR和CD80表达的影响。方法 :从健康献血员血液中分离单核细胞并进行体外培养 ,分别以M CSF和IL 10单独及共同作用 ,收集上清 ,用ELISA法检测单核细胞IL 12和IL 18的分泌 ,用流式细胞术检测细胞表面HLA DR及CD80的表达。结果 :①M CSF能诱导单核细胞分泌IL 18(P <0 .0 5 ) ;IL 10则能抑制IL 18的产生 (P <0 .0 5 ) ,并拮抗M CSF增强LPS诱生IL 18的作用 (P <0 .0 5 )。M CSF和IL 10均能抑制单核细胞分泌IL 12 p4 0 (P <0 .0 5 ) ,并具有协同效应 (P <0 .0 5 )。②M CSF能诱导单核细胞表面HLA DR的表达 (P <0 .0 5 ) ;IL 10则可抑制HLA DR的表达 ,并拮抗M CSF对HLA DR的诱导作用。M CSF对CD80表达的影响不大 ,IL 10能促进CD80的表达。结论 :M CSF和IL - 10可通过对单核细胞分泌IL 12、IL 18及HLA DR和CD80表达的调节 ,影响T细胞的活化、分化及其介导的免疫应答

The effects of M-CSF and IL-10 on IL-12 and IL- 18 production and expressions of HLA-DR and CD80 by human monocytes

AIM: To explore the effects of M-CSF and IL-10 on IL-12 and IL-18 production and expressions of HLA-DR and CD80 by human peripheral blood monocytes. METHODS: Human monocytes were isolated from blood of healthy donors, and cultured with M-CSF and or IL-10 alone or together. Then the monocytes and culture supernatant were collected respectively. IL-12 p40 and IL-18 levels in culture supernatant were detected by ELISA and the expressions of CD80 and HLA-DR on the monocytes were analyzed by FACS. RESULTS: (1)M-CSF induced secretion of IL-18 by the monocytes, while IL-10 inhibited the production of IL-18 and antagonized enhancement of LPS-induced IL-18 production by M-CSF. Both M-CSF and IL-10 inhibited secretion of IL-12 p40 by the monocytes and had a synegistic effect in the inhibition. (2)M-CSF could induce while IL-10 inhibited HLA-DR expression. Moreover, IL-10 had an antagonistic action on HLA-DR expression induced by M-CSF. M-CSF had no influence on CD80 expression, while IL-10 induced CD80 expression. CONCLUSION: M-CSF and IL-10 can regulate IL-12 and IL-18 production and HLA-DR and CD80 expressions by human monocytes, which may affect the activation and differentiation of T cells and T cell-mediated immune response.