类风湿关节炎患者外周血单核细胞Toll样受体2的表达及意义

目的观察类风湿关节炎(RA)患者外周血单核细胞Toll样受体2(TLR2)的表达变化与疾病活动程度和临床指标的关系,探讨RA的发病机制。方法27例活动期RA患者、20例非活动期RA患者及18名正常对照,采用流式细胞术检测外周血单核细胞表面TLR2的表达,反转录聚合酶链反应(RT-PCR)检测细胞内TLR2 mRNA的表达。结果活动期RA患者外周血单核细胞TLR2的表达水平显著高于缓解期RA患者及正常对照,而缓解期RA患者TLR2与正常对照相比,差异无统计学意义。RA患者外周血单核细胞TLR2的表达与疾病活动评分(DAS)、血清C反应蛋白(CRP)及血沉(ESR)相关,而与类风湿因子(RF)及抗环瓜氨酸肽(抗CCP)抗体无明显相关性。结论活动期RA患者外周血单核细胞TLR2表达增高,且TLR2的表达与疾病活动指标密切相关。活动期RA患者存在着固有免疫系统的活化,TLR2的高表达可能促进了外周血单核细胞的活化。

Expression and significance of Toll-like receptor 2 in peripheral blood monocytes of rheumatoid arthritis patients

Objective To examine the expression of toll-like receptor 2(TLR2)in peripheral blood monocytes and explore its association with disease stages and clinical manifestations and to explore the patho- genesis of rheumatoid arthritis(RA).Methods The expression of toll-like receptor 2 in peripheral blood monocytes from 47 RA patientis(27 in active stage and 20 in stable stage)and 18 normal individuals were de- tected by flowcytometry and RT-PCR.Results The expression of toll-like receptor 2 in peripheral blood monocytes in patients with active disease was significantly increased compared to non-active patients and nor- mal individuals,The expression was found to correlate with the Disease Active Score(DAS),serum C-reactive protein(CRP)level and the erythrocyte sedimentation rate(ESR),but not correlate with rheumatoid factor (RF)and the anti-cyclic citrullinated peptide(CCP)antibody.Conclusion The expression of toll-like recep- tor 2 in peripheral blood monocytes of patients with active RA is significantly increased.And the expression is correlated with disease activity index.The innate immune system is activated in patients with active disease. And the increased expression may promote the activities of monocytes.