| 合成肽作抗原酶免疫技术检测非甲－戊型肝炎病人血清中抗HGV |
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| 引用本文： | 程云,郭仁锋,张建宗,曹阳,貌盼勇,朱传琳,王业东,罗清华,李伯安,傅体全,马洪滨,李静,辛少杰,程炳立,姜平,陶凌,乔小江,何江英. 合成肽作抗原酶免疫技术检测非甲－戊型肝炎病人血清中抗HGV[J]. 细胞与分子免疫学杂志, 1996, 0(3) |
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| 作者姓名： | 程云 郭仁锋 张建宗 曹阳 貌盼勇 朱传琳 王业东 罗清华 李伯安 傅体全 马洪滨 李静 辛少杰 程炳立 姜平 陶凌 乔小江 何江英 |
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| 作者单位： | 北京解放军传染病研究所免疫研究室 |
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| 摘 要： | 本文应用人工合成肽作为抗原建立了检测庚型肝炎病毒（ＨＧＶ）抗体的酶免疫技术（ＥＩＡ），最适抗原包被浓度为４μｇ／ｍｌ。在特异性验证的基础上，检测了３４例非甲－戊５型肝炎病人血清和３２例丙型肝炎感染病人血清。结果表明，在ＨＧＶ感染高危人群中，诸如输血后肝炎－丙型肝炎中有较高的ＨＧＶ的感染率。本方法的建立为临床上难于作出诊断的庚型肝炎提供了敏感、特异、易于应用的新技术。
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| 关 键 词： | 抗HGV；合成肽；EIA；非甲－戊型肝炎 |
Enzyme Immunoassay for Detection of Antibody to Hepatitis G Virus in Non-A-E Hepatitis sera Based on Synthetic Peptides |
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| Cheng Yun,Guo Renfeng,Zhang Jianzhong,et al.. Enzyme Immunoassay for Detection of Antibody to Hepatitis G Virus in Non-A-E Hepatitis sera Based on Synthetic Peptides[J]. Chinese journal of cellular and molecular immunology, 1996, 0(3) |
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| Authors: | Cheng Yun Guo Renfeng Zhang Jianzhong et al. |
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| Abstract: | Five synthetic peptides were prepared based on the amino acid sequence of hepatitis G virus(HGV) and were used to develope an enzyme immunoassay(EIA) for detection of the antiHGV in sera. Some of the approaches were employed to optimize the sensitivity and the specificity for the EIA. 34 sera from the non-A-E hepatitis patients and 32 sera from the hepatitis C patients were detected and compared by coated different synthetic peptide GP1-4 respectively and mixed in EIA. The results were shown that a synthetic peptide-based EIA is sensitive, specific and simple for detecting anti-HGV in the sera from patients with HGV infection. |
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| Keywords: | anti-HGV EIA synthetic peptides non-A-E hepatitis |
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