灵芝多糖对兔失血性休克再灌注血液流变学和脂质过氧化的影响

目的:探讨灵芝多糖对家兔失血性休克再灌注损伤时血液流变学和脂质过氧化的影响及可能机制.方法:将16只家兔复制成失血性休克模型后随机分为生理盐水再灌注组和灵芝多糖再灌注组,观察休克和再灌注前后血浆脂质过氧化产物丙二醛(MDA)含量、红细胞超氧化物歧化酶(SOD)活性、全血黏度、血浆黏度和红细胞变形能力的变化,以及灵芝多糖对上述指标的影响.结果:与休克前相比,休克时血浆MDA含量、全血黏度、血浆黏度明显升高(P均<0.05),红细胞SOD活性和变形能力则明显降低(P均<0.05).生理盐水再灌注时可明显降低休克时血浆黏度(P<0.05),但对全血黏度无影响(P>0.05);而红细胞SOD活性和变形能力与休克时比较则进一步降低(P<0.05),血浆MDA含量明显升高(P<0.05).灵芝多糖再灌注时全血黏度、血浆黏度与休克时相比均降低(P均<0.05),与生理盐水组比较,红细胞SOD活性和红细胞变形能力均升高(P均<0.05),血浆MDA含量明显降低(P<0.05),全血黏度降低(P<0.05).结论:灵芝多糖可明显改善失血性休克再灌注时的血液流变学特性,改善微循环,其效果优于生理盐水;而机制可能与灵芝多糖提高红细胞SOD活性、降低血浆MDA含量、抑制血液脂质过氧化增强有关.

Effects of ganoderma lucidum polysaccharide on hemorrheology and lipid peroxide in hemorrhagic shock/reperfusion injury in rabbits

Objective: To explore the effect and the mechanism of ganoderma lucidum polysaccharide(GLP) on hemorrheology and lipid peroxide in hemorrhagic shock /reperfusion injury. Methods: Sixteen (rabbits) were randomly divided into normal saline group and GLP group after replicating hemorrhagic shock/reperfusion model. The plasma malondialdehyde (MDA) contents, the superoxide dismutase (SOD) activity of erythrocyte, blood viscosity, plasma viscosity and erythrocyte deformability were observed before and after hemorrhagic shock/reperfusion. The effects of GLP on these parameters were also observed. Results: The (contents) of MDA, blood viscosity, plasma viscosity were increased significantly (all P<0.05) in the (course) of shock compared with those before shock, but the erythrocyte SOD activity and the erythrocyte (deformability) were decreased significantly (all P<0.05). With reperfusion of normal saline, the plasma (viscosity) was (decreased) significantly (P<0.05) but blood viscosity did not changed markedly compared with those in shock course, the erythrocyte SOD activity and the erythrocyte deformability were decreased (significantly) ((all P<0.05)), and the MDA content was increased (P<0.05). With treatment of GLP, the (abnormal) (changes) of all the above parameters were straightened up remarkably (all P<0.05) except the (plasma) (viscosity). (Conclusion): GLP has obviously protective effect on hemorrhagic shock/reperfusion injury (through) (improving) the (feature) of hemorrheology and its effect is better than normal saline. The (mechanism) of (improving) (hemorrheology) is (possibly) related to advancing erythrocyte SOD activity, (reducing) (plasma) MDA (contents) and (inhibiting) the blood lipid peroxide.