Molecular Genetic Analysis of the ABO Blood Group System: 1. Weak Subgroups: A3 and B3 Alleles期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Molecular Genetic Analysis of the ABO Blood Group System: 1. Weak Subgroups: A³ and B³ Alleles

Authors:	Fumi-ichiro Yamamoto PhD Patricia D. McNeil Miyako Yamamoto Sen-itiroh Hakornori Teresa Harris W. John Judd and Robertson D. Davenport

Affiliation:	The Biomembrane Institute and University of Washington, Seattle, Wash.;Pacific Northwest Regional Blood Services, Portland, Oreg.;Department of Immunohematology, The University of Michigan Hospitals, Ann Arbor, Mich, USA;Department of Pathology, The University of Michigan Hospitals, Ann Arbor, Mich, USA

Abstract:	We have determined the nucleotide sequences of the coding region in the last two coding exom of ABO genes (which occupy 91% of the soluble form of A¹ transferase) from 7 individuals with weak subgroup phenotypes. Four of the individuals had an A₃ phenotype and 3 individuals had a B₃ phenotype. We determined the nucleotide sequences based on PCR followed by subcloning and DNA sequencing of the amplified fragments. Two cases of the A³ allele and 1 case of the B³ allele were found to contain a single-base substitution which resulted in an amino acid substitution. However, no other cases of A³ and B³ alleles were found to contain differences in this region. This finding demonstrates for the first time heterogeneity among these weak subgroups at the nucleotide level.

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