用ROC曲线比较TRFIA和RIA检测AFP在HCC诊断中的价值

分别用时间分辨荧光免疫分析(TRFIA)和RIA法检测血清AFP浓度,比较两种检测方法对原发性肝细胞癌(HCC)诊断的临床应用价值.选择门诊和住院期间得到确诊的原发性肝癌患者118例和肝良性病变患者123例,分别用TRFIA和RIA法检测受试者血清AFP浓度,并行工作特征(receiver operating characteristic,ROC)曲线分析.以正常对照组95%区间为医学正常值范围,TRFIA法和RIA法诊断肝癌的临床界限值分别为7.2ng/mL和23.2ng/mL.TRFIA诊断肝癌的灵敏度为45.8%,特异性为92.4%,诊断准确率为75.9%.RIA法的灵敏度为40.7%,特异性为91.4%,诊断准确率为67.6%.两种检测方法ROC曲线下面积分别为0.759(TRFIA)和0.676(RIA).TRFIA和RIA法都可以满足临床AFP检测需要,但TRFIA法明显优于RIA法.

Application of ROC Curve in the Evaluation of the Diagnostic Value of AFP Determined by TRFIA and RIA in HCC

To determine the levels of AFP in serum by Time-resolved Abstract:To evaluate the clinical value of the time-resolved immunofluoroassay(TRFIA) and radioimmunoassay(RIA) methods in the determination of serum AFP levels for the diagnosis of hepatocellular carcinoma(HCC),one hundred and eighteen patients with HCC and 123 patients with liver benign disease(LBD) were included in this study.The serum levels of AFP were determined by TRFIA and RIA,respectively.The results from serum samples from the patients with HCC and LBD groups were used to calculate receiver operating characteristic curves(ROC).The cut off values of serum AFP levels for TRFIA and RIA were set at 7.23 ng/mL and 23.20 ng/mL,respectively.The diagnostic specificity and sensitivity were 45.8% and 91.4% for TRFIA,and 40.7% and 91.47% for RIA respectively.The area under the ROC curve for TRFIA and RIA was 75.9% and 65.5%,respectively.The results indicate that both TRFIA and RIA could meet the clinical diagnostic needs and TRFIA is superior to RIA for identifying patients with HCC.