| 破骨细胞对多孔碳酸钙陶瓷材料物降解的实验研究 |
| |
| 引用本文： | 杨帆,谭金海,邢林卿,熊亮,雷鹏程. 破骨细胞对多孔碳酸钙陶瓷材料物降解的实验研究[J]. 武汉大学学报(医学版), 2009, 30(2): 159-162,封2 |
| |
| 作者姓名： | 杨帆 谭金海 邢林卿 熊亮 雷鹏程 |
| |
| 作者单位： | 武汉大学中南医院骨科,湖北,武汉,430071;武汉大学中南医院骨科,湖北,武汉,430071;武汉大学中南医院骨科,湖北,武汉,430071;武汉大学中南医院骨科,湖北,武汉,430071;武汉大学中南医院骨科,湖北,武汉,430071 |
| |
| 基金项目： | 湖北省科技攻关项目(编号:2005AA301C17) |
| |
| 摘 要： | 目的:采用破骨细胞与多孔碳酸钙陶瓷(PCCC)体外混合培养方法,观察其对PCCC的降解作用。方法:从新生SD大鼠长骨骨髓中分离出破骨细胞,与PCCC材料混合培养,分别于48h及96h终止培养并观察。结果:在倒置显微镜下见破骨细胞体呈圆形或椭圆形,多核,胞质伸出许多细微突起。抗酒石酸酸性磷酸酶(TRAP)染色阳性。扫描式电子显微镜观察见在PCCC表面出现许多散在分布的吸收空隙,直径一般50μm,破骨细胞位于其中。96h见吸收空隙增大。结论:破骨细胞在体外培养条件下对PCCC材料有明显吸收降解作用。
|
| 关 键 词： | 降解 破骨细胞 多孔碳酸钙陶瓷 |
Biodegradation Effects of Osteoclasts on Porous Calcium Carbonate Ceramics in Vitro |
| |
| YANG Fan,TAN Jinhai,XING Linqing,XIONG Liang,LEI Pengcheng. Biodegradation Effects of Osteoclasts on Porous Calcium Carbonate Ceramics in Vitro[J]. Medical Journal of Wuhan University, 2009, 30(2): 159-162,封2 |
| |
| Authors: | YANG Fan TAN Jinhai XING Linqing XIONG Liang LEI Pengcheng |
| |
| Affiliation: | YANG Fan,TAN Jinhai,XING Linqing,XIONG Liang,LEI Pengcheng Dept.of Orthopedics,Zhongnan Hospital of Wuhan University,Wuhan 430071,China |
| |
| Abstract: | Objective: To observe the biodegradation of porous calcium carbonate ceramics(PCCC) through mixed culture of osteoclasts and PCCC in vitro.Methods: Osteoclasts were isolated from neonatal SD rats' marrow of long bone and cultured on PCCC.The culturing was ended separately at 48 h and 96 h.Results: Under inverted microscope,the osteoclasts presented round or oval bodies with multi-nuclei and many thin projections.These cells were stained positively with tartaric acid resistance acid phosphatase(TRAP).Scannin... |
| |
| Keywords: | Degradation Osteoclast Calcium Carbonate |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
