成釉细胞瘤的侵袭性生物学行为研究

目的　研究成釉细胞瘤 (AB)侵袭性生长的生物学行为 ,对其相关因素进行分析。方法　采用免疫组化SP法 ,对 4 3例AB(原发 16例 ,复发 2 1例 ,恶性 6例 )进行了上皮性钙黏附蛋白 (E cad)、基质金属蛋白酶 (MMP 2、MMP 9)及其血管生成因子 (VEGF)的定位表达。结果　侵袭恶变AB细胞离散度增加 ,基膜断裂消失 ,瘤细胞外侵 ,E cad在AB中表达下降 ,MMP 2、MMP 9在AB上皮中有 2 8/41例和 30 /43例强表达 ,AB中VEGF阳性表达随着AB复发、恶变而逐渐增加 ,伴随AB的复发与恶变E cad、MMP 9、VEGF与其生物学行为显著相关 (rs=0 30 9、0 5 19、0 381,P <0 0 5 )。结论　AB为具有较高侵袭性的肿瘤 ,其生物学行为与E cad的丢失和异常表达 ,MMP 2、MMP 9、VEGF的强表达有关

The study of the invasive biologic behavior of ameloblastoma

Objective To investigate the invasive biologic behavior of ameloblastoma (AB) and to analyze its correlative factors Methods The specimens of 43 cases of AB (primary AB 16 cases, recurrent AB 21 cases, malignant AB 6 cases) were examined immunohistochemically using the streptavidin biotin method to determine the expression of E cadherin(E cad), matrix metalloproteinase(MMP) 2, MMP 9 and vascular endothelial growth factor (VEGF) Results The cells in malingant AB scattered more, grew invasively, and the basal membrane ruptured or lost The expression of E cad in AB descended, MMP 2 and MMP 9 were strongly expressed in the epithelia cells of 28/41, 30/43 cases of AB, respectively The positive rate and intensity of VEGF increased as AB recurred and transformed malignantly The expression of E cad, MMP 9 and VEGF were related to rucurrence or malignant transformation of AB (r s =0 309, 0 519, 0 381, P <0 05) Conclusion AB is a high invasive tumor The biological behavior of AB is related to lost or abnormal expression of E cad, the high expression of MMP 2, MMP 9, and VEGF