<Emphasis Type="Italic">Blastocystis hominis</Emphasis>: neutral red supravital staining and its application to in vitro drug sensitivity testing |
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| Authors: | Alexandre A Vdovenko John E Williams |
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| Institution: | (1) London School of Hygiene and Tropical Medicine, Gower Street, London WCIE 7HT, UK, GB;(2) University of Hawaii, Dept. of Pathology, 1960 East-West Road, T-512C, Honolulu, HI 96822, USA e-mail: Vdovenko@aol.com Tel.: +1-808-3950075; Fax: +1-808-9565465, US |
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| Abstract: | A simple in vitro drug sensitivity testing system for Blastocystis hominis clinical isolates was developed. Application of supravital staining by neutral red allowed quantitative viability assessment.
Four xenic cultures, isolated from human sources, were grown in modified monophasic Robinson's medium and tested for sensitivities
to nine available drugs. Assessment was done using the cell-count method from air-dried preparations after supravital staining
with neutral red. Also, the light absorbence method was evaluated. Trimethoprim, metronidazole, quinacrine, tetracycline,
paromomycin, and two new antiprotozoal drugs, nitazoxanide and deacetyl-nitazoxanide, showed cytostatic or cytocidal effects
on all or some Blastocystis isolates. Chloroquine and sulphamethoxazole did not demonstrate any effect at the concentrations studied.
Received: 20 November 1998 / Received last revision: 27 December 1999 / Accepted: 29 December 1999 |
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