Inhibiting ERp29 expression enhances radiosensitivity in human nasopharyngeal carcinoma cell lines |
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Authors: | Lin Qi Ping Wu Xin Zhang Yuanzheng Qiu Weihong Jiang Donghai Huang Yong Liu Pingqing Tan Yongquan Tian |
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Institution: | (1) Department of Otolaryngology, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha, 410008, Hunan Province, People’s Republic of China; |
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Abstract: | ERp29 is an endoplasmic reticulum (ER) stress-inducible protein. It was found that ERp29 was highly expressed in several cancers
and associated with resistance to oxidative and radiation stress, which may serve as a novel target for nasopharyngeal carcinoma
(NPC) anticancer approach. In this study, we used immunohistochemistry to detect ERp29 expression in radioresistant and radiosensitive
NPC tissues. As a result, ERp29 was up-regulated in radioresistant NPC tissues compared to radiosensitive NPC tissues. We
also found that ERp29 knockdown attenuated radioresistance of NPC CNE-1 cells and ERp29 overexpression enhanced radioresistance
of NPC CNE-2 cells. When exposed to radiation, ERp29 knockdown CNE-1 cells increased radiation-induced cell apoptosis and
ERp29 overexpression CNE-2 cells reduced radiation-induced cell apoptosis. Further, we demonstrated that ERp29 up-regulated
the expression of Hsp27. In conclusion, our study supports ERp29 could potentiate resistance to radiation in NPC cells, targeting
of ERp29 is a rational strategy in treating radioresistant NPC. |
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