Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells

Objective:To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma(HCC) cells in vitro.Methods:Total saponin was extracted from the root of A.valvata(TSAVD).HCC cells,such as BEL-7402,HepG2,PLC,SMMC-7721,MHCC-97-H, and MHCC-97-L,were treated with TSAVD in 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide(MTT) assay.BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays,and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed.Meanwhile,the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. Results:TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios(IRs) of 61.08%,74.12%, 84.55%at 24,48,and 72 h,respectively.Meanwhile,TSAVD inhibited MHCC-97-H proliferation in a concentrationdependent manner from 1.5 to 0.5 mg/mL,with the IR of 36%at 1.5 mg/mL at 24 h.For SMMC-7721,PLC, and HepG2,the IR was lower than 30%at 1.5 mg/mL at 24 h.In the wound healing assay,mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group.After pretreatment for 24 h with TSAVD,adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells,with IRs of 48.50%±4.86%and 49.85%±5.25%at 200 |xg/mL.The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91%and 79.37%±0.09%at 200μg/mL In the invasion assay,IRs were 69.78%±4.88%and 82.48%±0.25%at 200μg/mL Conclusions:Of all HCC cells,the highest inhibition by TSAVD was seen for BEL-7402 proliferation.TSAVD could restrain adhesion,invasion,mobility,and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.