Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats |
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引用本文: | 杨叔禹,赵能江,李学军,张惠杰,陈可冀,李灿东.Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats[J].Chinese Journal of Integrated Traditional and Western Medicine,2012,18(4):262-268. |
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作者姓名: | 杨叔禹 赵能江 李学军 张惠杰 陈可冀 李灿东 |
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作者单位: | Post-Graduate School,Fujian University of Traditional Chinese Medicine;Department of Internal Medicine,the First Affiliated Hospital of Xiamen University;Xiamen Diabetes Institute,Xiamen,Fujian Province 361003,China |
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基金项目: | Supported by the National Natural Science Foundation of China(No.30973912);the Innovation Project of Fujian Provincial Health Bureau(No.2007-CXB-10);the Project of Technology Bureau of Xiamen City(No.3502Z20077050) |
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摘 要: | Objective:To investigate the therapeutic effects of Ping-tang Recipe(平糖方,PTR) on highfat diet(HFD)-induced insulin resistance and non-alcoholic fatty liver disease(NAFLD),and to elucidate the underlying mechanisms.Methods:Forty male SD rats were included in the study.Ten rats were fed on normal diet as normal control,and thirty rats were fed on HFD for 8 weeks to induce obesity,followed with low dose (0.42 g/kg) or high dose(0.84 g/kg) of PTR or vehicle for 8 weeks with 10 animals for each group.Glucose metabolism and insulin sensitivity were evaluated by oral glucose tolerance test and insulin tolerance test. Hepatic steatosis was measured by immunohistochemistry.Liver lipid metabolic genes were analyzed by quantitative real-time polymerase chain reaction,while AMP-activated protein kinase(AMPK) expression was examined by Western blot.Results:Rats fed on HFD developed abdominal obesity,insulin resistance and NAFLD.PTR treatment reduced visceral fat(peri-epididymal and peri-renal) accumulation,improved glucose metabolism,and attenuated hepatic steatosis.The expressions of the key lipolytic regulating genes,including peroxisome proliferators-activated receptorγco-activator 1α(PGC-1α),peroxisome proliferator-activated receptorγ(PRAR-γ) andα(PRAR-α),were up-regulated(P<0.05 or P<0.01),while the expressions of lipogenic genes such as sterol regulatory element-binding protein 1c(SREBP-1c),fatty acid synthase (FAS) and liver fatty acid-binding protein(L-FABP) were down-regulated(P<0.05 or P<0.01).In addition, PTR activated AMPK and promoted acetyl-CoA carboxylase phosphorylation in the liver.Conclusions:PTR improves insulin resistance and reverse hepatic steatosis in the rat model of HFD-induced obesity through promotion of lipolysis and reduction of lipogenesis,which involves the AMPK signaling pathway,thus representing a new therapeutic intervention for obesity related insulin resistance and NAFLD.
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关 键 词: | Ping-tang Recipe non-alcoholic fatty liver disease insulin resistance AMP-activated protein kinase |
Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats |
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Authors: | Shu-yu Yang Neng-jiang Zhao Xue-jun Li Hui-jie Zhang Ke-ji Chen and Can-dong Li |
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Institution: | 1.Post-Graduate School,Fujian University of Traditional Chinese Medicine,Fuzhou(350108),China;2.Department of Internal Medicine,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian Province(361003),China;3.Xiamen Diabetes Institute,Xiamen,Fujian Province(361003),China |
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Abstract: | Objective To investigate the therapeutic effects of Ping-tang Recipe (平糖方, PTR) on high-fat diet (HFD)-induced insulin resistance and
non-alcoholic fatty liver disease (NAFLD), and to elucidate the underlying mechanisms. |
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Keywords: | Ping-tang Recipe non-alcoholic fatty liver disease insulin resistance AMP-activated protein kinase |
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