| 肾脏花生四烯酸CYP羟化酶参与高血压的形成及维持正常血压的稳定 |
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| 作者姓名： | Zhang F Qian JQ Wang DW |
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| 作者单位： | 华中科技大学同济医学院附属同济医院内科基因治疗中心,华中科技大学同济医学院药理教研室 武汉,中国 430030 |
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| 基金项目： | Project supported by the National Natural Science Foundation of China, № 39870307. |
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| 摘 要： | 目的:研究肾脏特异性表达CYP4A1与血压变化的关系.方法:将含有开放阅读框的CYP4A1 cDNA克隆到真核表达载体pcDNA3.1,构建4Al/pcDNA3.1和反义4Al/pcDNA3.1重组体,舌下静脉注射转染SD雄性大鼠,经尾动脉测量收缩压.用Western blot及Northern blot分析转染对照pcDNA3.1质粒及正、反义CYP4A1重组体后,CYP4A1在大鼠的脑、心、肺、肝、肾组织表达水平.结果:转染正义、反义CYP4A1两周后,大鼠的血压与对照组相比分别增加1.8kPa±0.3kPa(13.2mmHg±2.5mmHg)和减少了1.7kPa±0.3kPa(13.0mmHg±2.2 mmHg).Western blot及Northern blot结果均显示CYP4A1可选择性地在肾脏表达,而在脑、心、肺、肝几乎无表达,且在给予反义CYP4A1的大鼠肾脏中CYP4Al蛋白表达几乎被完全阻断.结论:在正常的SD大鼠肾脏中,转染正、反义CYP4A1可引起血压升高和降低,说明肾脏花生四烯酸CYP羟化酶参与高血压的形成及维持正常血压的稳定.
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| 关 键 词： | 花生四烯酸 一元酚单氧化酶 羟化酶 细胞色素P-450 CYP4A1 高血压 20-羟花生四烯酸 肾 |
Arachidonate CYP hydroxylases of kidney contribute to formation of hypertension and maintenance of blood pressure |
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| Zhang F,Qian JQ,Wang DW.Arachidonate CYP hydroxylases of kidney contribute to formation of hypertension and maintenance of blood pressure[J].Acta Pharmacologica Sinica,2002,23(6):497-502. |
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| Authors: | Zhang Fan Qian Jia-Qing Wang Dao-Wen |
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| Institution: | Department of Internal Medicine and Gene Therapy Center, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. |
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| Abstract: | |
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| Keywords: | arachidonic acid monophenol mono- oxygenase hydroxylases cytochrome P-450 CYP4A1 hypertension 20-hydroxyeicosatetraenoic acids kidney |
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