| 中线T细胞淋巴瘤的TCR—γ基因重排研究 |
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| 引用本文： | Chen D,Liu W,Liu Y. 中线T细胞淋巴瘤的TCR—γ基因重排研究[J]. 中华病理学杂志, 1998, 27(3): 198-201 |
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| 作者姓名： | Chen D Liu W Liu Y |
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| 作者单位： | 华西医科大学第一附属医院病理科 |
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| 基金项目： | 国家自然科学基金,美国中华医学基金 |
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| 摘 要： | 目的对中线Ｔ细胞淋巴瘤的克隆性进行研究。方法用一组针对Ｔ细胞受体γ链基因Ｖ区片段的家族特异性引物和ＰＣＲ方法,对１１例（２２个标本）中线Ｔ细胞淋巴瘤病例进行了Ｔ细胞受体γ基因重排的检测。结果２２个标本中２１个有Ｔ细胞受体γ链基因的克隆性重排（９４４５％）。在９例原发灶的连续活检和１例原发灶及其转移灶的标本中均未发现增生的克隆家族的改变。结论中线Ｔ细胞淋巴瘤的病变组织中存在Ｔ细胞的单克隆性增生,为该肿瘤的Ｔ细胞起源提供了分子生物学的证据。在中线Ｔ细胞淋巴瘤的疾病过程中（包括转移灶）增生Ｔ细胞的家族未发生变化,支持肿瘤的单克隆起源学说
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| 关 键 词： | 淋巴瘤.T细胞 聚合酶链反应 受体.抗原.T细胞 基因重排.T淋巴细胞 |
Detection of T-cell receptor gamma chain gene rearrangement in midline T-cell lymphomas by PCR method |
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| Chen D,Liu W,Liu Y. Detection of T-cell receptor gamma chain gene rearrangement in midline T-cell lymphomas by PCR method[J]. Chinese Journal of Pathology, 1998, 27(3): 198-201 |
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| Authors: | Chen D Liu W Liu Y |
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| Affiliation: | Department of Pathology, West China University of Medical Sciences, Chengdu 610041. |
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| Abstract: | OBJECTIVE: To investigate the clonality of midline T-cell lymphoma. METHODS: Using polymerase chain reaction method and a panel of family specific primers for the variable regions of TCR-gamma gene, and totally 11 cases (22 samples) of midline T-cell lymphoma (midline malignant reticulosis) were studied. RESULTS: Family specific monoclonal TCR-gamma gene rearrangements were found in 21 out of 22 samples (94.45%). There was no change of clonality during the course of disease. CONCLUSION: There is monoclonal T-cell proliferation in midline T-cell lymphoma and the clonality is not transformed with the advance of the disease. This finding provides an evidence to support the hypothesis of monoclonal origin of neoplasm. |
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| Keywords: | Lymphoma T cell Polymerase chain reaction Receptors antigen T cell Gene rearrangement T lymphocyte |
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