p38 MAPK在小鼠睾丸出生后不同发育阶段的表达

目的：探讨p38 MAPK在小鼠睾丸出生后不同发育阶段的表达．方法：应用免疫组织化学SABC法检测1～7周龄小鼠睾丸p38MAPK的表达和定位．结果：在2周龄小鼠睾丸生精小管上皮中即可观察到p38MAPK免疫阳性反应，免疫反应阳性细胞为精原细胞；3，4和5周龄小鼠睾丸仅有个别生精小管上皮可见p38 MAPK免疫阳性反应；6，7周龄小鼠睾丸中p38 MAPK表达较丰富，免疫反应阳性细胞为精原细胞和初级精母细胞，免疫阳性反应物均主要位于细胞核内．在7周龄小鼠睾丸中还可见到部分间质细胞呈p38 MAPK阳性，免疫阳性反应物主要位于细胞质内．结论：p38 MAPK可能对生精细胞的增殖分化以及雄激素的分泌具有调控作用。

Expression of p38 MAPK in mouse testis during postnatal development

AIM: To study the expression of p38 MAPK in mouse testis during postnatal development. METHODS: Immunohistochemical SABC method was used to detect the expression of p38 MAPK in 1 to 7 week old mice testes. RESULTS: The immunostaining of p38 MAPK was seen in spermatogonium of 2 week old mice testes. Only a few seminiferous tubules were p38 MAPK positive in 3 to 5 week old mice, whereas the expression of p38 MAPK was abundant in the testes of 6 and 7 week old mice. The immunolocalization of p38 MAPK was in spermatogonium and primary spermatocyte with positive substance in nucleus. In addition, some Leydig cells were p38 MAPK positive in the testes of 7 week old mice with positive substance in cytoplasm. CONCLUSION: p38 MAPK may play a role in the regulation of spermatogenic cell proliferation and androgen secretion.