体外培养的神经球中表达神经巢蛋白细胞的流式细胞术检测

采用体外培养不同时间的神经干细胞球 ,间接荧光法标记神经巢蛋白 (nestin) ,以流式细胞术检测神经干细胞球中表达nestin细胞的情况。结果显示 :在培养不同时间的细胞群体中均检出 nestin阳性细胞 ,培养 7d、1月、3月和 6月的细胞群含nestin阳性细胞数百分比分别为 (4 2 .2± 7.6) %、(4 1.7± 7.3 ) %、(3 3 .8± 12 .9) %和 (3 7.1± 5 .0 ) % ,几个时间点的细胞群体所含的 nestin阳性细胞数的百分比之间无显著性差异。提示 ,在体外培养条件下 ,神经干细胞群体可能通过自我更新和分化的调控 ,保持 nestin阳性细胞于一种较为恒定的比例。

DETERMINATION OF THE CELLS EXPRESSING NESTIN IN NEUROSPHERES CULTURED IN VITRO BY FLOW CYTOMETRY

The cells expressing nestin in neural stem cell neurospheres that cultured in different time were determined by flow cytometry using in-directing immunofluorescence staining. The results showed that nestin positive cells could be measured in the neurospheres cultured in differerent time. The percentages of nestin positive cells were 42.2±7.6%, 41.7±7.3%, 33.8±12.9% and 37.1±5.0%, respectively in the cell populations cultured 7 days, 1 month, 3 months and 6 months. No significant difference was found in the cell populations with different culture time. It is suggested that neural stem cells may modulate their self-renewal and differentiation to keep cells expressing nestin in a relatively identical percentage under the culture condition.