不同产区黄芩SSR分子标记鉴别研究

目的 采用简单重复序列（SSR）分子标记技术鉴定不同产区黄芩种质资源。方法 基于黄芩基因组数据设计SSR引物，并对来自河北、东北（辽宁、吉林地区）、内蒙古、山东、山陕（山西和陕西）地区共计5个产区的黄芩进行遗传多样性分析及聚类分析。结果 引物组合Sb2、Sb16、Sb22、Sb23、Sb26可以对产地为辽宁、吉林的黄芩进行鉴定区分；引物组合Sb21、Sb22、Sb28可以对产地为内蒙古的黄芩进行鉴定区分；引物组合Sb5、Sb13、Sb16、Sb22可以对产地为陕西、山西的黄芩进行鉴定区分。结论 研究结果可为不同产区黄芩种质资源的鉴定提供一种准确、高效的技术方法。

SSR for Identification of Scutellaria baicalensis from Different Producing Areas

Objective To identify the germplasm resources of Scutellaria baicalensis Georgi from different regions.Methods Simple sequence repeats (SSR) primers were designed based on genome data of S. baicalensis, and genetic diversity analysis and cluster analysis were performed on S. baicalensis from 5 regions: Hebei, Northeast China (Liaoning, Jilin), Inner Mongolia, Shandong, Shanshaan (Shanxi and Shaanxi).Results The combination of primers Sb2, Sb16, Sb22, Sb23, and Sb26 can distinguish samples from Liaoning and Jilin, and primers Sb21, Sb22, and Sb28 together can identity samples from Inner Mongolia. In addition, the combination of Sb5, Sb13, Sb16, and Sb22 can distinguish samples from Shanxi and Shaanxi.Conclusion This study provides an accurate and efficient technical method for the identification of S. baicalensis from different regions.