Simple Separation Technique of Peripheral Blood Cells: A New Method of Centrifugal Subfractionation

Addition and flotation methods for preparation of platelet- and leucocyte-rich plasma (PLRP) were compared: Addition methods were found preferable to flotation methods due to higher yield of leucocytes (p < 0.01) and simpler technique. Inappropriate preparation of PLRP might occur in addition methods, due to partial coagulation, and in flotation methods, due to marked mixing. A new centrifuge tube is described which simplified the separation of polymorphonuclear leucocytes, lymphocytes, erythrocytes, and platelets.