流行性出血热发热后期免疫疗法疗效研究

目的为了研究联合抗过敏加用新鲜血浆疗法对流行性出血热(EHF)发热后期患者的疗效。方法确诊的EHF发热后期病人20例,用红细胞C3b受体花环及免疫复合物花环法检测红细胞免疫功能,用双抗体夹心法检测血清中可溶性白细胞介素-2受体(sIL-2R)水平,用PEG法检测循环免疫复合物(CIC)水平,用免疫酶法检测复合物溶解活性(CRA)水平。结果与正常对照组相比,EHF患者红细胞免疫复合物花环形成率、血清中SIL-2R和CIC水平上升,红细胞C3b受体花环形成率和CRA水平下降,差异均极显著。应用联合抗过敏加输新鲜血浆疗法治疗后,红细胞免疫复合物花环形成率、sIL-2R和CIC水平下降,红细胞C3b受体花环形成率和CRA水平上升,与治疗前相比差异均极显著。结论作者认为,对EHF发热后期患者应用此疗法有效,检测上述指标对患者诊断、治疗及预后评估均有指导意义。

A study on effect of immunological therapy used in treatment of late-stage epidemic hemorhagic fever

To investigate the effect of antiallergic therapy in combination with fresh plasma transfusion on late-stage epidemic hemorrhagic fever (EHF) . Methods A total of 20 patients with EHF at the late stage were investigated.The red-cell immune function was studied by RBC C3b receptor rosette and immune complex rosette tests, level of soluble inter-leukin-2 receptor (sIL-2R) analyzed by ELISA, level of circulating immune comlex (CIC) detected by PEG method and level of complement mediated release activity (CRA) determined by immune enzyme method. Results Compared with the control group, the ratio of RBC immune complex rosette, the level of sIL-2R and CIC in the serum of EHF patients were elevated but the ratio of RBNC C3b receptor rosette and the level of CRA decreased in them. After the treatment with antiallergic therapy in combination with fresh plasma transfusion, the ratio of RBC immune complex rosette, the level of sIL-2R and CIC were reduced, while the ratio of RBC C3b receptor rosette and the level of CRA increased. Conclusion The treatment is effective for EHF and the determination of red-cell immune function, sIL-2R, CIC and CRA may be valuable for diagnosis and treatment of EHF.