| 怀山药试管块茎形成过程中内源激素的变化 |
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| 引用本文： | 常莉,薛建平,宋运贤,盛玮,熊聪慧.怀山药试管块茎形成过程中内源激素的变化[J].中国中药杂志,2010,35(21):2818-2821. |
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| 作者姓名： | 常莉 薛建平 宋运贤 盛玮 熊聪慧 |
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| 作者单位： | 淮北师范大学生命科学学院资源植物生物学安徽省重点实验室,安徽,淮北,235000 |
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| 基金项目： | 资源植物生物学安徽省重点实验室项目(KLPRB20 0913) |
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| 摘 要： | 目的:通过分析5种内源激素GA3,IAA,ABA,ZR和JA在山药试管块茎形成建成中的动态变化,初步探讨山药试管块茎形成的生理生化机制。方法:山药试管苗在试管块茎诱导培养基MS+6-BA 1.5 mg.L-1+NAA 1.5 mg.L-1+蔗糖5%中培养,取不同生长期的小块茎,采用ELISA法对其内源激素进行分离、纯化、测定。结果:在山药试管块茎形成过程中,GA3含量呈现倒"S"形变化;IAA含量总体呈下降趋势;ABA,ZR初期含量升高,后期下降;JA含量则一直上升;GA3与ABA和JA比值变化较为显著。结论:在山药试管块茎形成过程中,内源激素ABA,JA,IAA,ZR和GA3起着重要的调控作用。
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| 关 键 词： | 山药 试管块茎 内源激素 |
| 收稿时间： | 2010/4/30 0:00:00 |
Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro |
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| CHANG Li,XUE Jianping,SONG Yunxian,SHENG Wei and XIONG Conghui.Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro[J].China Journal of Chinese Materia Medica,2010,35(21):2818-2821. |
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| Authors: | CHANG Li XUE Jianping SONG Yunxian SHENG Wei and XIONG Conghui |
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| Institution: | School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China;School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China;School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China;School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China;School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China |
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| Abstract: | |
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| Keywords: | Dioscorea opposita microtuber endogenous hormones |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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