矿盐粉粒吸入疗法对哮喘小鼠肺组织病理形态及血清IL-4、IL-5、IL-13水平的影响

目的： 探讨天然矿盐粉粒吸入对哮喘小鼠肺组织病理及血清IL-4、IL-5、IL-13水平的影响。方法： 将40只Balb/c雌性小鼠随机分为对照组、模型组、30 min治疗组、60 min治疗组，每组10只。第0、7天分别在模型组、30 min治疗组、60 min治疗组小鼠腹腔注射致敏液鸡卵清蛋白（Ovalbumin,OVA），对照组注射生理盐水。第14天对模型组和治疗组行超声雾化吸入OVA以诱发哮喘；对照组则以生理盐水雾化吸入。第15天起，两治疗组每天分别用矿盐粉粒吸入治疗30 min或60 min，对照组和模型组则吸入空气，各组共吸入10 d。第22至第24天连续诱发哮喘3天，第25天处死小鼠，取肺组织常规行病理切片，ELISA法检测血清IL-4、IL-5、IL-13水平。结果： 与对照组比较，模型组小鼠肺组织病理出现支气管收缩、管腔狭窄、黏膜上皮坏死脱落，大量炎症细胞浸润、嗜酸性粒细胞聚集。与模型组比较，治疗组上述表现明显减轻。与对照组比较，模型组小鼠血清IL-4、IL-5和IL-13含量明显升高（P＜0.01或P＜0.05）；与模型组比较，两治疗组的IL-4、IL-5、IL-13含量则明显降低(P＜0.01)。结论： 吸入天然矿盐粉可减轻支气管哮喘小鼠气道炎症，降低血清中哮喘相关细胞因子IL-4、IL-5、IL-13的含量。

Impact of natural salt powder inhalation treatment in asthmatic mice to the pathological changes of lung and blood serum levels of IL-4,IL-5 and IL-13

Objective: To investigate the pathological changes of lung and the influence to serum IL-4,IL-5 and IL-13 levels in asthmatic mice treated by salt powder inhalation.Methods: Forty Balb/c female mice were divided randomly into control group,model group,therapy group A,therapy group B with each group of 10 only.On day 0 and 7,the mice of model group,therapy group A and therapy group B were injected intra-peritoneal with liquid sensitized(ovalbumin),while the mice of control group with saline.On day 14,the mice inhaled the aerosol of 1% ovalbumin to induce asthma for the first time.On day 15,therapy group A and therapy group B were treated with salt powder inhalation for 30 min and 60 min every day,respectively.The control group and the model group were treated with air inhalation.On day 22,23,24,the asthma symptoms were induced with the same method continuously.On day 25,all mice were put into death.The lungs of mice were observed by pathological method.The levels of serum IL-4,IL-5,IL-13 were tested by ELISA.Results: Compared with control group,the lungs of model group mice had bronchial constriction,mucosal epithelial necrosis,inflammatory cells infiltration with eosinophils gathered.Compared with model group,the therapy group A and therapy group B improved markedly.The levels of serum IL-4,IL-5 of model group were higher than those of the control group obviously(P<0.01).And the level of IL-13 of model group was high too(P<0.05).The levels of IL-4,IL-5 and IL-13 of therapy group A and therapy group B were lower than those of model group significantly(P<0.01).Conclusion: Inhaling natural salt powder could alleviate airway inflammation of bronchial asthma mice,and reduce the levels of related cytokines IL-4,IL-5 and IL-13 in serum.