Molecular cloning, tissue expression, and chromosomal assignment of a novel gene encoding a subunit of the human signal-recognition particle |
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Authors: | H Harada H Nagai N Mine Y Terada H Fujiwara I Mikami M Tsuneizumi A Yabe K Miyazaki T Yokota I Imoto J Inazawa M Emi |
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Institution: | (1) Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki 211-8533, Japan Tel. +81-44-733-5230; Fax +81-44-733-5192 e-mail: memi@nms.ac.jp, JP;(2) Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan, JP |
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Abstract: | Human cancers derived from breast, esophageal, or ovarian tissues frequently show allelic losses on chromosome band 17q25.
Moreover, a locus responsible for hereditary focal nonepidermolytic palmoplantar keratoderma, a condition associated with
esophageal cancer (TOC; tylosis with oesophageal cancer), has been mapped to the same band. During efforts to sequence, by
shotgun methods, a 1-Mb target region that we had defined as the DNA segment harboring the putative tumor suppressor gene(s)
involved in these events, we identified a novel cDNA. The full-length cDNA is 2495 bp long and is expressed predominantly
in skeletal muscle, heart, kidney, and placenta. The predicted product, a 627-amino-acid protein, exhibited significant sequence
homology to the canine 68-kd subunit of the signal recognition particle that has been implicated in the transport of secreted
and membrane proteins to the endoplasmic reticulum for proper processing. We confirmed the location of this gene at chromosome
17q25.1 by radiation-hybrid mapping and by fluorescence in situ hybridization.
Received: September 18, 2000 / Accepted: November 10, 2000 |
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Keywords: | Signal-recognition particle 17q25 Tylosis with oesophageal cancer Breast cancer hSRP68 |
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