| 高效液相色谱法测定苯磺酸氨氯地平的含量 |
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| 引用本文： | 汤韶坛,谢佳琪,苟帮润,陈小明. 高效液相色谱法测定苯磺酸氨氯地平的含量[J]. 中国药业, 2014, 0(23): 57-58 |
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| 作者姓名： | 汤韶坛 谢佳琪 苟帮润 陈小明 |
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| 作者单位： | 湘潭大学化学学院环境友好化学与应用教育部重点实验室 |
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| 基金项目： | 湖南省科技厅重点项目,项目编号：2013SK2021; 湖南省研究生科研创新项目,项目编号：CX2012B251 |
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| 摘 要： | 目的建立药物中苯磺酸氨氯地平的含量测定的方法。方法采用高效液相色谱法,色谱柱为Wonda Sil C18柱（150 mm×4.6 mm,5μm）,流动相为甲醇-0.03 mol/L乙酸铵溶液（70∶30）,流速为0.8 mL/min,柱温为室温,检测波长为237 nm。结果苯磺酸氨氯地平质量浓度在2-1 000μg/mL范围内与峰面积线性关系良好（r=0.999 9）。平均加样回收率为99.49%,RSD为1.35%（n=9）。结论该方法简便、准确、线性范围宽,重复性好,可作为药物中苯磺酸氨氯地平含量测定的方法。
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| 关 键 词： | 苯磺酸氨氯地平 含量测定 高效液相色谱法 |
Content Determination of Amlodipine Besylate by RP-HPLC |
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| Tang Shaotan,Xie Jiaqi,Gou Bangrun,Chen Xiaoming. Content Determination of Amlodipine Besylate by RP-HPLC[J]. China Pharmaceuticals, 2014, 0(23): 57-58 |
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| Authors: | Tang Shaotan Xie Jiaqi Gou Bangrun Chen Xiaoming |
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| Affiliation: | (Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Huaan, China 411105) |
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| Abstract: | Objective To establish a RP- HPLC method for the content determination of amlodipine besylate in drugs. Methods The analysis was accomplished on the Wonda Sil C18 column（ 150 mm × 4. 6 mm, 5 μm）, with the mobile phase of methanol- 0. 03 mol / L ammonium acetate solution（ 70 ∶ 30）, at room temperature, the UV detection wavelength of 237 nm and a flow rate of 0. 8 mL / min.Results Amlodipine besylate in the concentration range of 2- 1 000 μg / mL exhibited a good linear relationship with the peak area（ r = 0. 999 9）. The average recovery rate of the sample size was 99. 49%, RSD = 1. 35%（ n = 9）. Conclusion This method is simple and accurate with wide linear range and good reproducibility,and can be used to determine the content of amlodipine besylate in drugs. |
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| Keywords: | amlodipine besylate content determination HPLC |
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