经皮瘤内注射32P玻璃微球对肝癌组织的影响

目的探讨经皮瘤内注射~(32)P玻璃微球(~(32)P-GMS)对肝癌瘤组织的影响及其最佳剂量。方法建立24只兔VX2肝癌模型,分为A、B、C、D 4组并分别经皮瘤内注射37、74、111和148个放射性强度单位(MBq)的~(32)P-GMS,7、14和21 d后分批处死实验兔并取注药部位肿瘤组织,在光镜和电镜下观察其病理变化。结果注射~(32)P-GMS剂量与肿瘤细胞的放射性损害相关。A、B组不能完全杀死癌组织；C组肿瘤细胞几乎完全消失,纤维结缔组织对瘤灶形成完整的包绕；D组未见存活的肿瘤组织,但见正常肝组织亦被累及。结论经皮瘤内注射适量的~(32)P-GMS可以最大限度地杀伤瘤组织,本实验显示111 MBg为最佳剂量,对于阻止肝癌转移具有重要意义。

Influence on the tumor after percutaneous intra-tumor injection of 32P-GMS in liver cancer

Objective To study the influence on the tumor after percutaneous intra-tumor injection of ~(32)P-GMS in liver cancer as well as its suitable dose.Methods 24 New Zealand rabbits were used to establish the animal model of VX-2 liver cancer,and divided into A,B,C and D groups with individually 37,74,111 and 148 MBq of ~(32)P-GMS being injected,respectively;and then pathological changes of tumor were observed by light and electron microscope respectively.Result The dose of ~(32)P-GMS was obviously correlated with the radioactivity damage of tumor cells.In the A and B groups,the tumor cells were not observed to disappear completely after injection of ~(32)P-GMS,but in C group,tumor cells were almost completely disappeared and surrounded by a lot of connective tissue.Although the tumor cells were found to disappear completely in D group,normal liver tissues were also involved.Conclusion Percutaneous intra-tumor injection of ~(32)P-GMS with suitable dose that may induce the tumor tissue to be maximally damaged and may also provide some significances to prevent the tumor metastasis.