IL-6、IL-1和PDGF对大鼠肾小球系膜细胞生长的影响

目的：探讨白细胞介素－６（ＩＬ－６）、白细胞介素－１（ＩＬ－１）和血小板源生长因子（ＰＤＧＦ）对大鼠肾小球系膜细胞（ＭｓＣ）增殖的影响。方法：采用改良的ＭＴＴ法和ＢｒｄＵ掺入法检测ＳＤ大鼠体外ＭｓＣ悬液中加入ＩＬ－６、ＩＬ－１及ＰＤＧＦ后１２ｈ及２４ｈ的增殖情况。分７组进行观察。结果：ＩＬ－６组、ＩＬ－１组和ＰＤＧＦ组１２ｈ及２４ｈ的光密度（ＯＤ值）与标记指数（ＬＩ）均为２％ＦＣＳ组也均高，但所有实验组均不能达２０％ＦＣＳ组的细胞增殖水平。联合应用双因子刺激１２ｈ及２４ｈ均未见明显的协同促增殖作用。结论：ＩＬ－６、ＩＬ－１及ＰＤＧＦ均能在一定程度上刺激大鼠肾小球ＭｓＣ增生。联合应用双因子刺激无明显的协同效应。

Effect of IL-6,IL-1 and PDGF on the growth of rat mesangial cells in vitro

Purpose:To study the effect of IL 6,IL 1 and PDGF on the growth of rat glomerular mesangial cells (MsC) in vitro.Methods:Improved MTT method and BrdU incorporation method for the detection of MsC growth on 12 h and 24 h after stimulation of IL 6,IL 1 and PDGF.Results: After singly stimulating MsC in vitro by IL 6, IL 1 and PDGF, the OD value an LI are both higher than that in 2% FCS. Combinely using IL 6 and IL 1 or IL 6 and PDGF, the OD value and LI are both higher than that in 2% FCS in 12 h and 24 h, but never higher than the value in 20% FCS. Combinely using IL 6 and IL 1 or IL 6 and PDGF, there were no significant synergic effect.Conclusions: It is suggested IL 6, IL 1 and PDGF could stimulate the proliferation of cultured MsC. Combinely using IL 6 and IL 1 or IL 6 and PDGF, there were no significant synergistic effect.