香加皮提取物抗肿瘤活性的研究

背景与目的： 研究中药香加皮提取物的抗肿瘤活性，探讨其抗肿瘤机制。 材料与方法： 采用噻唑蓝(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide, MTT)法观察香加皮醇提物和水提物对MCF-7、TE-13、QG56、SMMC7721、T24、Hela、K562等肿瘤细胞增殖的抑制作用，应用流式细胞术检测肿瘤细胞的周期变化和对凋亡的影响。 结果： 香加皮醇提物和水提物均能明显抑制多种肿瘤细胞的增殖，呈浓度依赖性；醇提物的抑瘤作用(IC50<10 μg/ml)强于水提物(IC50<40 μg/ml)，并且差异具有统计学意义(P<0.05)。香加皮醇提物可将MCF-7细胞阻滞于G0/G1期，并呈时间依赖性地诱导MCF-7细胞发生凋亡。10 μg/ml香加皮醇提物作用MCF-7细胞24 h，与对照组相比，G0/G1期细胞显著增多，作用48 h，凋亡率从对照组的0.70％±0.13％升高到13.54％±2.12％，两者之间的差异有统计学意义(P<0.05)。 结论： 香加皮提取物对体外肿瘤细胞的增殖具有显著的抑制作用，醇提物强于水提物，其抗瘤机制可能与阻滞肿瘤细胞周期和诱导凋亡有关。

Anti-tumor Activity of Extract from Periploca Sepium Bge

BACKGROUND ＆ AIM: To investigate the anti-tumor activity of extract from Periploca Sepium Bge (PSBE) and to study its probable mechanisms. MATERIALS AND METHODS: The growth-inhibitory effect of ethanol extract from Periploca sepium bge (PSBEE) and water extract from Periploca sepium Bge (PSBWE) on MCF-7,TE-13,QG56,SMMC7721,T24,Hela,K562 tumor cell lines were examined by using MTT assay, the change of cell cycle and the induction of apoptosis were analyzed with flow cytometry. RESULTS: Both PSBEE and PSBWE could significantly inhibit the proliferation of tumor cell lines, the effect was dose-dependent, PSBEE (IC50<10 μg/ml) was more effective than PSBWE (IC50<40 μg/ml). PSBEE could arrest the cell cycle at G0/G1 phase and induce apoptosis in MCF-7 cells in a time-dependent manner. The proportion of tumor cells in the G0/G1 phase increased after treatment with 10 μg/ml PSBEE for 24 hours, the apoptotic rate reached 13.54％±2.12％ after 48 h. CONCLUSION: Cell growth in vitro was inhibited significanttly by extract from Periploca sepium Bge, PSBEE might exert its anti-tumor activity by blocking the cell cycle and inducing apoptosis of tumor cells.