| PCR-PAGE用于Y染色体微缺失的检测 |
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| 引用本文： | 张红岩,刘肖曼,赵晶,徐俐,王雪倩. PCR-PAGE用于Y染色体微缺失的检测[J]. 中国计划生育学杂志, 2007, 15(6): 364-365 |
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| 作者姓名： | 张红岩 刘肖曼 赵晶 徐俐 王雪倩 |
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| 作者单位： | 青岛市计划生育研究所,266071 |
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| 摘 要： | 目的:建立PCR-PAGE分子生物学技术,从基因水平检测导致男性不育的Y染色体微缺失。方法:采用多重PCR技术及PAGE分离技术对男性不育患者的Y染色体AZF区域进行检测。结果:51例男性不育患者中有5例AZF区域存在缺失,缺失率为9.8%。结论:PCR-PAGE分子生物学检测Y染色体微缺失技术有效、简便,适用于男性不育患者的诊断。
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| 关 键 词： | Y染色体微缺失 AZF STS 多重PCR PAGE |
| 收稿时间： | 2007-02-13 |
| 修稿时间： | 2007-02-132007-04-16 |
Determination of Y Chromosome Microdeletion by PCR - PAGE |
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| Zhang Hongyan, Liu Xiaoman, Zhao Jing,et al.. Determination of Y Chromosome Microdeletion by PCR - PAGE[J]. Chinese Journal of Family Planning, 2007, 15(6): 364-365 |
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| Authors: | Zhang Hongyan Liu Xiaoman Zhao Jing et al. |
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| Affiliation: | Qingdao Institute for Family Planning in Shandong Province, Qingdao 266071 |
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| Abstract: | Objective:To establish a molecular biology technique of PCR-PAGE to determine Y chromosome microdeletion which could be a important factor in male infertility at a gene level.Methods:Multiple PCR method and PAGE technology were used to detect azoospermia factor(AZF)regions of Y chromosome.Results:5 cases existed microdeletion among 51 infertile males,the prevalence rate of microdeletion was 9.8%.Conclusion:The PCR-PAGE method is effective and simple in determination of Y chromosome microdeletion,and is applicable for diagnosis of male infertility. |
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| Keywords: | AZF STS PAGE |
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