用聚合酶链反应检测单疱病毒性角膜炎静止期角膜内HSV基因

目的:单疱病毒性角膜炎是主要的致盲眼病之一,近年来研究认为HSV可在角膜内潜伏,但未获结论性证据,本研究目的为明确角膜是否为HSV的另一潜伏地。方法:选择18例已确认为HSK病例,所有病例角膜病变静止3个月以上(平均3.7年),行部分穿透性角膜移植术取下角膜片,用聚合酶链反应方法(引物为P15’CATCACCGACC-CGGAGAGGGAC,P2 5’GGGCCAGGCGCTTGGTGTA)检测角膜片内HSV基因。结果:18例静止期HSK病例角膜片中,用聚合酶链反应方法在17例病例中检测出HSV基因,1例阴性。结论:单疱病毒性角膜炎静止期角膜内有HSV基因存留,角膜可能为HSV的另一潜伏地,用聚合酶链反应检测角膜内HSV DNA方法简单,敏感性高。眼科学报 1995;11:183—185。

Detection of Herpes Viral Genome in Corneal Buttons of Quiescet Herpes Simplex Keratitis with Polymerase Chain Reaction

Purpose: Herpetic keratitis is one of the major cause of blindness. The recent reports have proposed that the herpetic viruses may persist in cornea but provided noconclusive evidence. This study is to determine whether the cornea is another latent site of herpes simplex virus.Methods: 18 corneal buttons of patients with quiescent herpes simplex keratitis which had quited for at least three months (mean 3. 7 years) and were obtained by corneal transplantation were detected using polymerase chain reaction (PCR). The sequences of the primers are: P15'CATCACCGACCCGGAGAGGG-AC, P25'GGGCCAGGCGCTTGTTGGTGTA.Results: The positive HSV DNA were found in 17 corneal buttons out of 18 cases.Conclusion: The HSV genomes are present in the cornea of quiescent herpetic keratitis , which suggest that the cornea may be another latent site of herpetic virus. The PCR is a simple, specific and sensitive way to detect HSV genomes in cornea. Eye Science 1995; 11: 183-185.