上皮性钙粘蛋白与肝细胞癌侵袭、转移的关系研究

目的探讨上皮性钙粘蛋白(E-Cadherin)在人肝细胞癌(HCC)中的表达及其与肝癌侵袭、转移性的关系。方法选择56例有完整随访资料的原发性肝细胞癌及相应癌旁组织标本、20例正常肝组织标本,用 RT-PCR 方法检测 E-Cad-herin mRNA 的表达;用免疫组织化学方法检测 E-Cadherin 的表达。结果 1.E-Cadherin 在肝细胞癌组织中的表达低于癌旁组织和正常组织,差异有显著性意义(P<0.05);癌旁组织和正常组织中 E-Cadherin 的表达无显著性差异(P>0.05);2.E-Cadherin 在肝癌组织中的表达与术后复发时间呈正相关(P<0.05;R~2=0.786),与病理分期呈负相关(P<0.05;R~2=0.678);3.癌组织中 E-Cadherin 表达与肝外转移呈负相关(P<0.05;R~2=0.704);4.癌旁组织中 E-Cadherin 表达与术后复发时间呈正相关(P<0.05;R~2=0.742)。结论 E-Cadherin 与肿瘤的分化程度,侵袭转移能力,复发倾向相关,在临床中有望作为肿瘤分化、复发、转移的评估指标之一。

Relationship Between E- Cadherin and Invasion and Metastasis of Hepatocellular Carcinoma

Objective To investigate the association between E-cadherin expression of hepatocellular carcinoma(HCC)and its invasion and metastasis.Methods RT-PCR and immunohistochemical analysis technology were used to detect E-cadherin mRNA expression and E- cadherin protein expression respectively in 56 paired HCC and non-HCC liver tissue samples and 20 normal liver tissue samples.Results 1. The level of E-cadherin expression in HCC was lower than that in adjacent non-HCC liver tissue and in normal liver tissue and the difference was statistically significant(P<0.05);the difference of E-cadherin expression between adjacent non-HCC liver tissue and normal liver tissue had no statistically significance(P>0.05);2.E-cadherin expression in HCC had a positive correlation with recurrence after surgical treatment (P<0.05,R~2=0.786)and a inverse correlation with pathological grade(P<0.05,R~2=0.678);3.There was an inverse correlation between E-cadherin expression in HCC and extrahepatic metastasis(P<0.05,R~2=0.704);4.Significant positive correlations were found between E -cadherin expression in adjacent non-HCC liver tissue and recurrence after surgieal treatment(P<0.05,R~2=0.742).Conclusion E- cadherin had an association with degree of tumor differentiation,capability of invasion and metastasis and tendency of recurrence.It is expected that E-cadherin will be one of indexes of differentiation,recurrence and metastasis.