微囊化转NGF基因NIH3T3细胞填充静脉修复大鼠坐骨神经缺损的作用

目的：了解微囊化转NGF基因NIH3T3细胞在修复周围神经缺损中的作用。方法：SD雄性大白鼠30只，建立大鼠坐骨神经离断模型后随机分为3组，A组：异体静脉桥接神经缺损，形成神经再生室,其内填充微囊化转NGF基因NIH3T3细胞，修复大鼠坐骨神经10 mm缺失；B组：自体神经移植；C组：微囊化NIH3T3细胞。于术后4、8及12周进行足迹试验，术后12周进行电生理学测试及形态学观察。结果：术后4、8和12周不同时间点坐骨神经功能指数，术后12周神经传导速度、再生神经的组织学改变及再生神经纤维的成熟程度A组和B组比较，差异均无显著性(P>0.05)， A 组和B组明显优于C组 (P<0.05)。结论：聚赖氨酸/海藻酸钠(APA) 微胶囊与外周神经组织有良好的生物相容性；辅加微囊化转NGF基因NIH3T3细胞对修复缺损的神经具有良好的桥梁作用和促神经生长作用。

Experimental study on microencapsulated NGF expressing NIH3T3 cells on repair of peripheral nerve defect in rats

Objective To investigate the effects of microencapsulated NGF expressing NIH3T3 cells on the repair of peripheral nerve defect in rats.Methods Thirty SD male rats with 10 mm defect of sciatic nerve were randomly divided into three groups(n=10).Group A: The nerve defect was bridged with heterogeneous nerve,the neural regeneration room was formed and filled with microencapsulated NGF expressing NIH3T3 cells.Group B: Autogenous nerve grafting was performed.Group C: The nerve defect was bridged with heterogeneous nerve,the neural regeneration room was formed and filled with microencapsulated NIH3T3 cells.The walking track analysis,electrophysiological and morphological observation were performed 12 weeks after operation.Results There were no significant differences of sciatic nerve function index,nerve concluctive velocity,histological changes of regenerative nerve,maturation degree of regenerative nerve fiber between group A and group B 4,8,12 weeks after operation;group A and B were surperior to group C(P<0.05).Conclusion There is good biocompatibility between the microencapsules and peripheral nerve tissue of rat in vivo.The microencapsulated NGF expressing NIH3T3 cells is good bridge and can promote regeneration of peripheral nerve.