人宫颈癌致癌基因和Pokemon在肝细胞癌中的表达及其相关性

目的 观察人宫颈癌致癌基因(HCCR)和Pokemon在肝细胞癌中的表达水平,探讨两者间的关系.方法 应用实时定量逆转录-聚合酶链反应(RT-PCR)法检测41例肝癌组织、43例癌旁组织及其相应44例血液标本、3例肝硬化组织及其相应的3例血液标本以及3例正常肝组织中HCCR、Pokemon的表达水平,分析肝细胞癌(HCC)患者血液样本中HCCR、Pokemon表达水平与临床指标的关系.结果 肝癌、癌旁、肝硬化及正常肝组织HCCR阳性表达率分别为100.00%、97.67%、100.00%、100.00%;Pokemon阳性表达率分别为97.56%、97.67%、100.00%、100.00%.但HCCR、Pokemon在各类组织中的表达水平差异有统计学意义(P<0.05),肝细胞癌、癌旁、肝硬化、正常肝等组织中的表达水平呈递减趋势.肝细胞癌患者血液中HCCR、Pokemon阳性率分别为79.55%及50.00%.两者的表达水平显著相关.结论 HCCR和Pokemon可能可以做为HCC相关的肿瘤标记物.HCCR和Pokemon在肝细胞癌患者中存在某种程度的关系.

Expression of HCCR and Pokemon in hepatocelluar carcinoma and their relationship

Objective To detect and analyze the expression of HCCR and Pokemon in hepatocel-luar carcinoma (HCC) and their relationship. Methods The expression of Pokemon in all tissues inclu-ding blood (n=44), HCC tissues (n=41 ) and adjacent tissues ( n = 43 ), hepatocirrhosis ( n = 3 ) and normal liver tissues (n = 3 ) was detected by real-time PCR. The relationship between HCCR, Pokemon and clinical indications was analyzed. Results There was expression of HCCR and Pokemon in almost tis-sues,which showed significant difference. The expression levels of HCCR and Pokemon in hepatocelluar carcinomas, adjacent tissues, hepatocirrhosis, and normal liver tissues had a decreased trend. The positive rate of HCCR and Pokemon in blood of HCC was 79.55% and 50.00% ,respectively. The expression lev-els of HCCR and Pokemon had an obvious relationship in HCC patients. Conclusion HCCR and Poke-man maybe become marks of HCC. HCCR is relative to Pokemon to some exten in HCC patients.