高氧诱导支气管肺发育不良新生小鼠肺组织中lncRNA表达谱的变化

目的:分析长链非编码RNA (long non-coding RNA,lncRNA)在高氧诱导支气管肺发育不良(bronchopulmonary dysplasi,BPD)小鼠模型肺中表达谱的变化?方法:构建高氧诱导支气管肺发育不良新生小鼠模型,利用lncRNA芯片技术检测正常小鼠肺及BPD小鼠肺组织中lncRNA表达谱的变化,经过对原始数据进行预处理,筛选出差异表达的lncRNA?结果:与对照组相比,模型组差异表达的lncRNA(差异倍数≥2倍且P < 0.05)共1 769条,其中882条上调,887条下调;5倍以上上调的共140条,下调的共71条;10倍以上上调的共28条,下调的有2条?结论:高氧诱导BPD发生时,肺组织中lncRNA的表达谱发生了显著变化;这些差异表达的lncRNA,可能参与了BPD发生?发展过程?

Study on expression profile of long non-coding RNA in lung tissues of newborn mice with hyperoxia-induced bronchopulmonary dysplasia

Objective:To analyze the expression profile variation of long non-coding RNA(lncRNA) in lung tissues of newborn mice with hyperoxia-induced bronchopulmonary dysplasia (BPD). Methods:We first successfully established a newborn mouse model of hyperoxia-induced BPD. We used lncRNA microarray technology to inspect differences of lncRNA expression profile between normal control lung and BPD lung,which helped us screen out the lncRNAs with differential expression after pretreatment of raw data. Results: Compared with the control group,there were 1 769 lncRNAs with differential expression which had more than 2-fold changes and significant differences (P < 0.05),of which 882 increased more than 2 times and 887 reduced more than 2 times;140 increased more than 5 times;71 reduced more than 5 times;28 increased more than 10 times and 2 reduced more than 10 times. Conclusion:lncRNA expression profile of hyperoxia-induced BPD was significantly changed in comparison with normal lung tissues. These lncRNAs with differential expression may be involved in the occurrence and development of BPD.