盐知母不同有效部位改善链脲佐菌素诱导2型糖尿病大鼠糖代谢的研究

目的探究盐知母不同溶剂萃取物对2型糖尿病大鼠糖代谢的影响,旨在寻找盐知母降血糖作用的有效部位。方法 SD雄性大鼠ig脂肪乳10 g/kg,1次/d。4周后,ip链脲佐菌素35 mg/kg,建立2型糖尿病模型。造模成功的大鼠按血糖值随机分为模型组、阿卡波糖(0.2 g/kg)组以及石油醚萃取物、氯仿萃取物、醋酸乙酯萃取物、正丁醇萃取和萃余物组,连续ig给药14 d。于给药7、14 d后测定空腹血糖值(FBG),末次给药后测定口服葡萄糖耐量(OGTT),眼眶取血测定糖化血红蛋白(HbAlc)、血清胰岛素(FINS),计算胰岛敏感指数(ISI)和Homa胰岛素抵抗(HOMA-IR)指数。结果与模型组比较,盐知母氯仿萃取物能够明显降低FBG、HbA1c、FINS、HOMA-IR指数,升高ISI,改善OGTT;盐知母醋酸乙酯萃取物能够显著降低HbAlc、HOMA-IR指数,改善OGTT;盐知母正丁醇萃取物能够显著降低FINS;盐知母萃余物能够显著降低FBG、FINS、HOMA-IR指数,升高ISI。结论盐知母不同溶剂萃取物对2糖尿病大鼠糖代谢具有较好的改善作用,其中氯仿萃取物的作用最强。

Improvement of salt Anemarrhenae Rhizoma extracts on glucose metabolism in rats with type 2 diabetes mellitus induced by streptozotocin

Objective To explore the influence of various extracts from salt Anemarrhenae Rhizoma on glucose metabolism in rats with type 2 diabetes mellitus, and to find the effective part on the hypoglycemic effect. Methods Male SD rats were ig administered with fat milk 10 g/kg, once daily for 4 weeks, and established 2 type diabetic rat model by ip streptozotocin 35 mg/kg. Rats with type 2 diabetes mellitus were randomly divided into model group, acarbose (0.2 g/kg) group, and salt Anemarrhenae Rhizoma extracts groups of petroleum ether, chloroform, ethyl acetate, n-butanol, and raffinate. All animals were ig administered once daily for 14 d. Fasting blood glucose (FBG) was determined after treated for 7 and 14 d. After the last administration, the level of oral glucose tolerance test (OGTT) was determined. The blood samples were collected to measure the glycosylated hemoglobin (HbAlc) and fastings erum insulin (FINS), and insulin sensitivity index (ISI) and Homa insulin resistance (HOMA-IR) index were calculated. Results Compared with model group, chloroform extract group could significantly reduce the FBG, HbAlc, FINS, and HOMA IR index, increase ISI index and improve OGTT. Ethyl acetate extract could significantly reduce the HbAlc and HOMA IR index, and improve OGTT. n-Butanol extract could significantly reduce the content of FINS. Raffinate could significantly reduce the FBG, FINS, and HOMA IR index, and increase the ISI. Conclusion Various solvents extracts of salt Anemarrhenae Rhizoma can better improve glucose metabolism in rats with type 2 diabetes mellitus, and chloroform extract has stronger efficacy.