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| TNF-α含量的改变与肾小管细胞缺氧-复氧性损伤关系的研究 | |
| 引用本文： | 董文斌,杜逸亭,陈跃,航永伦,陈枫,邓存良. TNF-α含量的改变与肾小管细胞缺氧-复氧性损伤关系的研究[J]. 细胞与分子免疫学杂志, 2005, 21(6): 690-692 |
| 作者姓名： | 董文斌 杜逸亭 陈跃 航永伦 陈枫 邓存良 |
| 作者单位： | 1. 泸州医学院附属医院,新生儿科,四川,泸州,646000 2. 泸州医学院附属医院,核医学科,四川,泸州,646000 3. 泸州医学院附属医院,检验科,四川,泸州,646000 4. 泸州医学院附属医院,传染与免疫研究室,四川,泸州,646000 |
| 基金项目： | 四川省杰出青年学科带头人培养基金资助(No.04ZQ026-033)，四川省中医管理局重点科研基金资助(No.200239) |
| 摘 要： | 目的:探讨TNF-α含量的改变与肾小管上皮细胞株(HK-2)缺氧-复氧性损伤的关系。方法:以HK-2细胞株作为研究材料,采用液体石蜡覆盖法建立缺氧损伤模型,分别在缺氧4、12、24h和复氧4、12、24h,采用放射免疫分析法(RIA)、生化法和台盼蓝拒染法,检测培养液中TNF-α的含量、乳酸脱氢酶(LDH)的活性、细胞计数及活细胞率的改变。结果:缺氧后4h,TNF-α的含量、LDH的活性开始升高,细胞总数及活细胞计数开始下降,并于缺氧后24h达高峰。TNF-α含量的改变与LDH活性的升高呈显著的正相关(r=0.89,P<0.05),与活细胞计数的下降呈显著的负相关(r=-0.91,P<0.05)。结论:肾小管缺氧-复氧后,TNF-α的产生增多,并参与了肾小管细胞损伤的过程。 |
| 关 键 词： | 缺氧/复氧 TNF-α 肾小管上皮细胞 |
| 文章编号： | 1007-8738（2005）06-0690-03 |
| 收稿时间： | 2004-11-29 |
| 修稿时间： | 2005-07-04 |
The relationship between TNF-α and the injury of renal tubular cells caused by anoxia/reoxygenation |
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| DONG Wen-bin,DU Yi-ting,CHEN Yue,HANG Yong-lun,CHEN Feng,DENG Cun-liang. The relationship between TNF-α and the injury of renal tubular cells caused by anoxia/reoxygenation[J]. Chinese journal of cellular and molecular immunology, 2005, 21(6): 690-692 | |
| Authors: | DONG Wen-bin DU Yi-ting CHEN Yue HANG Yong-lun CHEN Feng DENG Cun-liang |
| Affiliation: | 1. Department of Newborn Medicine; 2.Department of Nuelear Medi- eine;3 .Department of Clinical Laborator; 4.Laboratory of Infection and Immunity, Affiliated Hospital of Luzhou Medieal College, Luzhou 646000, China |
| Abstract: | AIM: To investigate the relationship between TNF-alpha and renal tubular cell injury caused by anoxia/reoxygenation. METHODS: Human renal proximal tubular cell line HK-2 was used as model. Anoxia/reoxygenation were produced by covering/de-covering the cell culture with liquid paraffin wax. The level of TNF-alpha and the activity of lactate dehydrogenase(LDH) in the culture medium was determined by radioimmunoassay(RIA) and biochemical methods, respectively. Trypan blue exclusion was used to measure cell viability. RESULTS: Anoxia/reoxygenation could increase TNF-alpha level and LDH activity, but decrease viability of HK-2 cells. TNF-alpha level was positively correlated with LDH activity (r=0.89, P<0.05) and negatively with the cell viability (r=-0.91, P<0.05). CONCLUSION: TNF-alpha induced by anoxia/reoxygenation may participate in the process of renal tubular cell injury. |
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