白细胞介素-1B-511单核苷酸多态性与胃癌关系的流行病学研究

目的研究中国胃癌高、低发区白细胞介素（IL）-1B-511单核苷酸多态性、幽门螺杆菌（Hp）感染与胃癌的芙系。方法胃癌高发区（陕西省）胃癌患者、健康志愿者各102例，胃癌低发区（广东省）胃癌患者、健康志愿者各104例，两组人群在性别比及年龄上均匹配。采用限制性片段长度多态性（PCR—RFLP）分析IL-1B-511单核苷酸多态性，酶联免疫吸附法（ELISA）检测血清抗Hp—IgG抗体。结果在胃癌低发区，胃癌患者IL-1B-511T／T基因型频率明显高于对照人群（26．9％比13．4％，X^2=5．85，P〈0．05；OR=2．37，95％CI为1．16～4．82）。在胃癌高发区，胃癌患者IL-1B-511 T／T基因型频率与对照人群尢明显差异（27．5％比24．5％，X^2=0．41，P〉0．05）；高发区对照人群的IL-1B-511 T／T基因型频牢明显高于低发区相应人群（24．5％比13．4％，X^2=4．1，P〈0．05）。Hp感染轻度增加低发区人群发生胃癌的危险性（OR=3．03，95％CI为1．61～5．71），而IL-1B-511 T／T基因型增加Hp感染后胃癌发生的危险性（OR=8．0，95％CI为1．39～35．7）。结论IL-1B-511 T／T基因型与中国人胃癌发生有关，IL-1B-511 T／T基因型增加HP感染后胃癌发生的危险性。

Association of interleukin-1B-511 single nucleotide polymorphism and risk of gastric cancers in high and low prevalence regions in China

Objective To investigate the relationship between interleukin(IL)-1B-511 single nucleotide polymorphism,Helicobacter pylori(H.pylori) infection,and risk of gastric cancer in high prevalent (Shaanxi province) and low prevalent (Guangdong province) regions in China.Methods The study population consisted of age and gender-matched healthy volunteers and gastric cancer patients (102 volunteers and 102 gastric cancer patients from Shaanxi province,104 volunteers and 104 gastric cancer patients from Guangdong province).IL- 1B-511 single nucleotide polymorphism was analysed by PCR-restriction fragment length polymorphism (RFLP).H.pylori status of subjects was determined by an ELISA assay for serum anti-H,pylori IgG.Re- sults In low prevalence region.IL-1B-511 T/T genotype frequency was significantly higher in patients with gastric cancer than in control subjects (26.9% vs.13.4%,X~2=5.85,P<0.05,OR=2.37,95%CI:1.16- 4.82).In high prevalence region,IL-1B-511 T/T genotype frequency was no different between patients with gastric cancer and control subjects (27.5% vs.24.5%,X~2=0.41,P>0.05).Compared with the low prevalence region,control subjects from the high prevalence region had a higher frequency of the IL-1B-511T/T genotype (24.5% vs.13.4%,X~2=4.1,P<0.05).While H.pylori infection alone had a modest effect on the risk of gastric cancer (OR=3.03,95% CI:1.61-5.71) in the low prevalence region,IL-1B-511T/T genotype could increase cancer risk in H.pylori positive status (OR=8.0,95%CI:1.39-35.7).Conclusions IL-1B-511T/T genotype is associated with gastric cancer in China.The risk of developing gastric cancer is significantly increased for the T/T genotype with H.pylori infection.