IL-1B基因多态性在十二指肠溃疡发病中的作用

目的探讨幽门螺杆菌(Hp)感染与IL-1B-511位点单核苷酸多态性(SNP)在十二指肠溃疡(DU)发病中的作用。方法用病例-对照研究方法,调查某院54例DU患者及94例同院同期非DU患者(对照),用ELISA方法检测其血清Hp IgG抗体,PCR扩增IL-1B-511,限制片段长度多态性分析(RFLP)检测其SNP。结果DU组Hp阳性率为90.74%(49/54),而对照组则为54.26%(51/94),病例组高于对照组,OR为8.26(95%CI为3.02~22.59)。DU组IL-1B-511基因T/T、C/T和C/C基因型频率分别为31.48%,35.19%,33.33%;而在对照组则分别为38.30%,46.81%,14.89%,IL-1B-511 C/C型能增加DU的患病危险,OR为3.05(95%CI为1.23~7.57),但按有无Hp感染进行分层后,则IL-1B-511 C/C只在有Hp感染时才有作用,OR为4.60(95%CI为1.40~15.12)。结论Hp感染时IL-1B-511 C/C能增加十二指肠溃疡病的患病风险,机体的遗传基因与Hp共同影响Hp感染者的临床结局。

The role of interleukin-1 gene polymorphism in duodenal ulcer

Objective To examine H.pylori infection and function of interleukin-1-511 gene with duodenal ulcers(DU).Methods 54 patients with duodenal ulcer and 94 controls were recruited in case-control study.H.pylori infection was examined by ELISA and IL-1B-511 gene by PCR.Single-nucleotide polymorphism(SNP) was analyzed by PCR-RFLP.Results 90.74% of DU and 54.26% of controls were H.pylori positive and OR was 8.26(95% CI: 3.02-22.59),indicated that helicobacter pylori infection increased risk of duodenal ulcer.T/T,C/T and C/C genotype frequency of IL-1B-511 were 31.48%,35.19%,33.33% in DU and 38.30%,46.81%,14.89% in controls respectively.The OR of IL-1B-511 C/C was 3.05(95% CI: 1.23-7.57).After stratified by Hp infection,the OR of IL-1B-511 C/C was 4.60(95% CI:1.40-15.12).Conclusion IL-1B-511 C/C with H.pylori infection resulted in significant increases in susceptibility to duodenal ulcer,Host gene and Hp infection determined clinical outcome.