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Changes in mRNA of protein inhibitor of neuronal nitric oxide synthase following facial nerve transection |
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| Authors: | Che Y H Tamatani M Yamashita T Gomi F Ogawa S Tohyama M |
| Institution: | a Department of Anatomy and Neuroscience, Osaka University Medical School, 2-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan b Department Orthopedic Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan c CREST, Japan Science and Technology, Japan |
| Abstract: | Protein inhibitor of neuronal nitric oxide synthase (PIN) is reported as the protein inhibiting neuronal nitric oxide synthase (nNOS) activity by preventing dimerization of nNOS. It was also reported that PIN inhibits the activity of all nitric oxide synthase (NOS) isozymes. We examined the effects of facial nerve transection on PIN mRNA and NOS expression by in situ hybridization for PIN mRNA and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) staining. PIN mRNA was initially expressed and transiently increased from 3 to 5 days and returned to the basal level at 7 days after axotomy in the motoneurons of the facial nucleus. NADPH-d-positive motoneurons were found from 7 days post-operation in the facial nucleus. These results suggest that PIN may interact with NOS from 7 days post-operation. |
| Keywords: | Protein inhibitor of neuronal nitric oxide synthase Facial nerve Axotomy In situ hybridization Rat |
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