Selective toxicity of ethacrynic acid towards lymphocytes of chronic lymphocytic leukemia in vitro. |
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| Authors: | P R Twentyman E Lambert M Müller J K Rees |
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| Affiliation: | Department of Clinical Oncology and Radiotherapeutics, University of Cambridge, UK. |
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| Abstract: | We have used the MTT colorimetric assay to determine the sensitivity to ethacrynic acid of lymphocytes from normal donors and of peripheral blood cells from leukaemia patients. Whereas normal lymphocytes and cells from acute or chronic myeloid leukaemia showed similar sensitivities (median inhibitory dose, ID50 = 20-22 microM), lymphocytes from chronic lymphocytic leukaemia patients were much more sensitive (ID50 = 6 microM). This result was found irrespective of the assay duration. |
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