| 反相高效液相色谱法测定小儿清热灌肠剂中6种成分的含量研究 |
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| 引用本文： | 许家鸾,王京霞. 反相高效液相色谱法测定小儿清热灌肠剂中6种成分的含量研究[J]. 中国现代应用药学, 1998, 15(5): 56-57 |
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| 作者姓名： | 许家鸾 王京霞 |
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| 作者单位： | 浙江省中医院,杭州,310006 |
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| 摘 要： | 目的:建立了测定小儿清热灌肠剂中6种成分含量的方法.方法:反相高效液相色谱法.以ODS(5μm,4.6mm×250mm)为色谱柱,流动相A:CH_3OH-H_2O-HCOOH(10:90:1),流动相B:CH_3OH-H_2O-HCOOH(80:20:1),检测波长为280nm.结果:柴胡皂甙A含量为0.4190,加样回收率为90.1% ±2.2%,n=6,RSD=2.4%,番泻甙A含量为0.7790,加样回收率为93.99%±3.8%,n=6,RSD=4.0%,苦参碱含量为0.1290,加样回收率为100.6%±2.7%,n=6,RSD=2.9%,大黄素甲醚含量为0.3390,加样回收率为95.8%±1.4%,n=6,RSD=1.5%,大黄酸含量为0.2590,加样回收率为91.3%±2.1%,n=6,RSD=2.3%,大黄素含量为0.3190,加样回收率为94.6%±3.3%,n=6,RSD=3.5%.结论:该方法简便,重现性好.
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| 关 键 词： | 高效液相色谱 小儿清热灌肠剂 化学成分 中成药 |
Determination of six constituents in Xiaoerqinre sausage by RP-HPLC |
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| Xu Jialuan(Xu JL,Wang Jinxia( Wang JX. Determination of six constituents in Xiaoerqinre sausage by RP-HPLC[J]. The Chinese Journal of Modern Applied Pharmacy, 1998, 15(5): 56-57 |
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| Authors: | Xu Jialuan(Xu JL Wang Jinxia( Wang JX |
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| Abstract: | OBJECTIVE: A reversed - phase HPLC method was established for determination of six constituents in Xiaoer.METHODS:A HPLC method was developed under the following conditions:flow rate:1.0mo/min,the UV detector was set at 280nm, the mobile phase A was metha |
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| Keywords: | RP-HPLC Xiaoerqinre Sausage six constituents |
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