| Calyculin A诱导染色体凝聚的方法探讨 |
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| 引用本文： | 朱巍 郑斯英 钟慎斌 曹建平 朱财英. Calyculin A诱导染色体凝聚的方法探讨[J]. 苏州大学学报(自然科学版), 2001, 21(5): 533-534 |
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| 作者姓名： | 朱巍 郑斯英 钟慎斌 曹建平 朱财英 |
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| 作者单位： | 朱巍（苏州大学核医学院放射生物学教研室,苏州,215007） 郑斯英（苏州大学核医学院放射生物学教研室,苏州,215007） 钟慎斌（苏州大学研究处,苏州,215006） 曹建平（苏州大学核医学院放射生物学教研室,苏州,215007） 朱财英（苏州大学核医学院放射生物学教研室,苏州,215007） |
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| 摘 要： | 目的探索用Calyculin A诱导外周血淋巴细胞染色体发生凝聚的最优化方法.方法采用L9(34)正交试验,对影响Calyculin A诱导染色体凝聚方法的3个主要影响因素(Calyculin A的终浓度,Calyculin A的处理时间,细胞培养时间)进行探讨.结果Calyculin A的终浓度,Calyculin A的处理时间,细胞培养时间不同水平的影响具有统计学意义(P＜0.01).结论淋巴细胞培养48h,培养结束前2h加入Calyculin A,Calyculin A终浓度为50nmol/L的实验组合可以获得高比例的分裂指数.
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| 关 键 词： | Calyculin A 染色体凝聚 正交试验 |
| 文章编号： | 1000-5749(2001)05-0533-02 |
| 修稿时间： | 2001-06-20 |
Study of the Methology on the Premature Chromosome Condensation Induced by Calyculin A |
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| ZHU Wei,ZHENG Si-ying,ZHONG Shen-bin,et al.. Study of the Methology on the Premature Chromosome Condensation Induced by Calyculin A[J]. Suzhou University Journal of Medical Science, 2001, 21(5): 533-534 |
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| Authors: | ZHU Wei ZHENG Si-ying ZHONG Shen-bin et al. |
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| Abstract: | Objective To explore the best method of PCC (premature chromosome condensation) induced by Calyculin A.Methods three main factors influcing the method of PCC induced by Calyculin A were evaluated with L 9(3 4) orthogonal design.Result It was shown by statistics analysis that there was significant diversification ( P <0.01) in both three main factors (the Calyculin A final density,the treatment time of Calyculin A,and the culture time).Conclusion The result is the best when the Calyculin A final density is 50nmol/L,the treatment time of Calyculin A 2h, and the culture time 48h. |
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| Keywords: | Calyculin A PCC Orthogonal design |
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