| 胆碱对离体大鼠心肌缺血/再灌注损伤的保护作用 |
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| 引用本文： | 王丽艳,刘艳,李丹露,单宏丽,吕延杰,杨宝峰.胆碱对离体大鼠心肌缺血/再灌注损伤的保护作用[J].中国药理学通报,2007,23(5):600-603. |
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| 作者姓名： | 王丽艳 刘艳 李丹露 单宏丽 吕延杰 杨宝峰 |
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| 作者单位： | 1. 哈尔滨医科大学药学院药理学教研室,黑龙江,哈尔滨,150081
2. 哈尔滨医科大学药学院药理学教研室,黑龙江,哈尔滨,150081;黑龙江省生物医药重点实验室·省部共建国家重点实验室培训基地,黑龙江,哈尔滨,150081 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;黑龙江省卫生厅科研项目;黑龙江省杰出青年科学基金 |
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| 摘 要： | 目的观察胆碱对离体大鼠心肌缺血/再灌注损伤的影响。方法采用Langendorff灌注系统,建立离体大鼠全心缺血/再灌注损伤模型。观察胆碱对复灌后冠脉流量、心肌组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和心肌梗死面积的影响。结果胆碱可明显增加缺血/再灌注后1、5、10min的冠脉流量,减少心肌组织MDA含量、提高SOD活性,缩小心肌梗死面积,与模型组比较差异均有显著性(P<0.05)。上述作用可部分的被选择性M3受体阻断剂4-di-phenylacetoxy-N-methylpiperidine-methiodide(4-DAMP)所逆转。结论胆碱对离体大鼠心肌缺血/再灌注损伤具有保护作用。
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| 关 键 词： | 胆碱 心肌 缺血/再灌注损伤 4-DAMP M3受体 |
| 文章编号： | 1001-1978（2007）05-0600-04 |
| 修稿时间： | 2006-11-15 |
Protective effect of choline on ischemia-reperfusion injury in isolated rat heart |
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| WANG Li-yan,LIU Yan,LI Dan-lu,SHAN Hong-li,L Yan-jie,YANG Bao-feng.Protective effect of choline on ischemia-reperfusion injury in isolated rat heart[J].Chinese Pharmacological Bulletin,2007,23(5):600-603. |
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| Authors: | WANG Li-yan LIU Yan LI Dan-lu SHAN Hong-li L Yan-jie YANG Bao-feng |
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| Institution: | 1. Dept of Pharmacology, Haerbin Medical University, 2. Biopharmaeeutical Key Laboratory of Heilongjiang Province Incubator of State Key Laboratory, Haerbin 150081, China |
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| Abstract: | Aim To study the influence of choline on ischemia-reperfusion injury (IRI) in isolated rat heart. Methods The isolated rat heart models of IRI were developed by linking the isolated rat heart to Langendorff perfusion system and 25 min of ischemia followed by reperfusion for 30 min. The outflow of coronary artery, the Maleic Dialdehyde (MDA) content and Superoxide Dismutase (SOD) activity were measured. Results In the presence of choline, the outflow of coronary artery (P<0.05 or P<0.01) and the SOD activity (P<0.01) were increased, and the MDA content was decreased after reperfusion (P<0.05), and the infarct size were also found to depress. The protective effects of choline on IRI could be reversed by 4-diphenylacetoxy-N-methylpiperidine-methiodide (4-DAMP), a selective antagonist of M3 receptor. Conclusion A protective effect of choline on myocardial ischemia-reperfusion injury in rats in vitro was observed. |
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| Keywords: | 4-DAMP |
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