| 人血管平滑肌细胞种植构建工程生物血管的实验研究 |
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| 引用本文： | 朱楚洪,应大君,糜建红.人血管平滑肌细胞种植构建工程生物血管的实验研究[J].生物医学工程学杂志,2005,22(4):695-697. |
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| 作者姓名： | 朱楚洪 应大君 糜建红 |
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| 作者单位： | 第三军医大学,解剖教研室,生物力学与组织工程教育部重点实验室,生物力学研究室,重庆,400038 |
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| 基金项目： | 国家自然科学基金资助项目(30472027)，全军十五青年基金项目(01Q098)，重庆市科学基金资助项目 |
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| 摘 要： | 了解猪血管去细胞后平滑肌细胞种植情况,为猪血管用于血管组织工程提供资料。取猪颈动脉,生物酶预处理猪血管,在自行设计制作的新型动力性生物反应器中,用原代培养的人平滑肌细胞种植在去细胞血管基质材料内,HE染色及银染检测平滑肌细胞种植效果。结果表明生物酶预处理血管后,HE染色及银染检测可见血管腔平滑肌细胞形态正常,沿血管长轴分布,提示经生物酶预处理的猪血管人平滑肌细胞能成功种植,可望构建实用的组织工程血管。
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| 关 键 词： | 猪血管 平滑肌细胞 组织工程 |
| 收稿时间： | 2002-12-31 |
| 修稿时间： | 2002-12-312003-05-16 |
Study of Human Smooth Muscle Cells Seeding in Biologic Blood Vessel Matrix |
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| Zhu Chuhong,Ying Dajun,Mi Jianhong.Study of Human Smooth Muscle Cells Seeding in Biologic Blood Vessel Matrix[J].Journal of Biomedical Engineering,2005,22(4):695-697. |
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| Authors: | Zhu Chuhong Ying Dajun Mi Jianhong |
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| Institution: | Department of Anatomy, Biomechanics Section under the Key Lab for Biomechanics & Tissue Engineering of Ministry of Education, The Third Military Medical University, Chongqing, China. |
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| Abstract: | To explore the human smooth muscle cells seeding in blood vessel of minor pig after trypsin treatment and provide data for xenotransplantation and for using pig vessel in tissue engineering. HE and silver stain were used for checking the smooth muscle cells seeding in acellular blood vessel. The results showed that the smooth muscle cells seeding succeeded and the smooth muscle cells were in normal morphological distribution. These demonstrate that the pig aorta can be used for smooth muscle cells seeding, and hence for constructing new vascular grafts. |
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| Keywords: | Pig blood vessel Smooth muscle cell Tissue engineering |
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