分离培养骨巨细胞瘤破骨样细胞的方法学研究

本文通过组织块贴壁法、机械分离法和酶消化法对骨巨细胞瘤的破骨样细胞进行分离、培养。通过比较，结果显示：酶消化法提纯程度最高，收获量较多；机械分离法居中，但操作简单，对原位杂交、免疫组织化学和骨吸收功能检测具有一定的应用价值；组织块培养法需要时间长，各细胞成分混杂，对研究各细胞成分之间的关系，有一定帮助

Methods of isolation and culturing osteoclast-like cells of giant cell tumour

We cultured the osteoclast like cells (OLC) from giant cell tumour of bone in vitro by three methods.Comparison of the methods shows that isolation of the OLC by trypsin can obtain the purest and greatest amount of OLC.Mechanical isolation is simple and useful for in situ hybridzation,immunohistochemistry and detection of bone resorption.Explant culture requires long time and contains various cells from giant cell tumour,which is helpful in studying the relationship between these cells.