| 昆布多糖硫酸酯化及其结构研究 |
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| 引用本文： | 邹明畅,何立巍,蔡宝昌,谢卫红. 昆布多糖硫酸酯化及其结构研究[J]. 河南中医学院学报, 2009, 24(2) |
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| 作者姓名： | 邹明畅 何立巍 蔡宝昌 谢卫红 |
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| 作者单位： | 邹明畅,Zou Mingchang(南京中医药大学,江苏,南京,210046;江苏大学附属人民医院,江苏,镇江,212002);何立巍,蔡宝昌,谢卫红,He Liwei,Cai Baochang,Xie Weihong(南京中医药大学,江苏,南京,210046) |
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| 基金项目： | 江苏省镇江市科学技术局资助项目 |
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| 摘 要： | 目的:对昆布多糖进行硫酸酯化,并对产物的纯度与结构进行分析,为研究其生物活性奠定物质基础.方法:利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化,并使用HPLC-ELSD法测定反应产物的纯度与分子量;采用硫酸钡比浊法测定昆布多糖硫酸酯中SO2-4的含量,并利用波谱法研究其结构.结果:昆布多糖硫酸酯的纯度为96%,其分子量为16315,SO2-4含量为41.30%;紫外、红外光谱分析结果均显示硫酸酯化多糖的光谱特征,13C-NMR谱显示多糖的2位羟基为SO2-4的主要取代位置.结论:利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化,该方法所得产物的纯度和酯化率高.
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| 关 键 词： | 昆布多糖 硫酸酯化 昆布多糖硫酸酯 结构分析 |
Study on Preparation of LAMS from Laminarin and Its Structural Analysis |
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| Zou Mingchang,,He Liwei,Cai Baochang,Xie WeihongNanjing University of Chinese Medicine,Nanjing,Jiangsu,China .Affiliated People Hospital of Jiangsu University,China. Study on Preparation of LAMS from Laminarin and Its Structural Analysis[J]. Journal of Henan University of Chinese Medicine, 2009, 24(2) |
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| Authors: | Zou Mingchang He Liwei Cai Baochang Xie WeihongNanjing University of Chinese Medicine Nanjing Jiangsu China .Affiliated People Hospital of Jiangsu University China |
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| Affiliation: | Zou Mingchang1,2,He Liwei1,Cai Baochang1,Xie Weihong1Nanjing University of Chinese Medicine,Nanjing,Jiangsu,China 210046 2.Affiliated People Hospital of Jiangsu University,China 212002 |
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| Abstract: | Objectives:To sulfate the laminarin,then analyze the purity and elucidate the structure of polysaccharide sulfate,so as to make a foundation for the further research about its biological activity.Methods:LAMS was prepared by chlorsulfonic acid-pyridine method,and the purity and molecular weight was analyzed by HPLC-ELSD.The content of SO42-in polysaccharide sulfate was determined by barium sulfate turbidimetry,then the structure of polysaccharide sulfate was elucidated by UV,IR and 13C-NMR.Results:The purit... |
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| Keywords: | laminarin sulfate esterification polysaccharide sulfate structural elucidation |
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