| 胰岛素对大鼠血管平滑肌细胞增殖及胶原蛋白合成的影响 |
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| 引用本文： | 王旭开,王燕,杨成明,付春江,张晔,何作云.胰岛素对大鼠血管平滑肌细胞增殖及胶原蛋白合成的影响[J].第三军医大学学报,2006,28(24):2416-2418. |
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| 作者姓名： | 王旭开 王燕 杨成明 付春江 张晔 何作云 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所心血管内科,重庆,400042;第三军医大学基础医学部医学遗传学教研室,重庆,400038;第三军医大学新桥医院心血管内科,全军心血管内科中心,重庆,400037 |
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| 摘 要： | 目的 观察胰岛素对分离培养的大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖及胶原蛋白合成的影响.方法 采用大鼠的腹主动脉血管平滑肌细胞分离培养,应用3H-TdR和3H-脯氨酸掺人方法检测胰岛素对大鼠VSMCs干预后,VSMCs内DNA合成以及胶原蛋白合成.结果 胰岛素可促进处于静止状态的大鼠VSMCs DNA及胶原蛋白的合成,并呈现明显的浓度依赖关系,在40 nmol/L的浓度时DNA及胶原蛋白的合成达到高峰,DNA及胶原蛋白分别处于36 h和48 h时合成最为显著.结论 胰岛素可明显促进培养的VSMCs增殖及胶原蛋白的合成.
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| 关 键 词： | 胰岛素 血管平滑肌细胞 胶原蛋白合成 |
| 文章编号： | 1000-5404（2006）24-2416-03 |
| 收稿时间： | 2005-10-21 |
| 修稿时间： | 2006-04-05 |
Effect of insulin on proliferation and collagen protein synthesis of rat vascular smooth muscle cells |
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| WANG Xu-kai,WANG Yan,YANG Cheng-ming,FU Chun-jiang,ZHANG Ye,HE Zuo-yu.Effect of insulin on proliferation and collagen protein synthesis of rat vascular smooth muscle cells[J].Acta Academiae Medicinae Militaris Tertiae,2006,28(24):2416-2418. |
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| Authors: | WANG Xu-kai WANG Yan YANG Cheng-ming FU Chun-jiang ZHANG Ye HE Zuo-yu |
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| Institution: | 1.Department of Cardiovasology, Institute of Surgery Research, Daping Hospital, Chongqing 400042; 2. Department of Moleculer Genetics, College of Medicine, Chongqing 400035; 3.Department of Cardiovascular Disenses, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China |
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| Abstract: | Objective To understand the role of insulin in the cytobiology of blood vessels.Methods The vascular smooth muscle cells(VSMCs)from SD rat were cultured and respectively treated with 1,10,20,30,40 nmol/L insulin for 24 h or 30 nmol/L insulin for 12,24,36,48 h,or with DMEM solution containing 10% FBS as blank control.DNA synthesis and collagen protein synthesis of rat VSMCs were detected by 3H-TdR incorporation and 3H-proline incorporation.Results Insulin could significantly promote DNA synthesis and collagen protein synthesis of quiescent rat VSMCs in a dose-dependent manner.DNA synthesis and collagen protein synthesis reached the peak when insulin concentration was 40 nmol/L,and showed significant difference at 36 h and 48 h with that of the blank control.Conclusion Insulin could promote proliferation and collagen protein synthesis of cultured rat VSMCs. |
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| Keywords: | insulin vascular smooth muscle cell collagen protein synthesis |
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