补肾活血液延缓雄性大鼠增龄性骨质疏松的研究

目的：评价补肾活血液延缓雄性大鼠骨衰老的作用。方法：40只SD雄性大鼠随机分为4组，每组10只，分别为24月龄本底对照组、27月龄增龄对照组、补肾活血液低剂量组和补肾活血液高剂量组，增龄对照组不做任何处理，低剂量组、高剂量组均自24月龄给药至27月龄。进行相应处理后分别测定各组大鼠全身骨矿物密度（GBMD）、右侧股骨抗弯强度（FBS）和血清骨钙素（BGP）、尿羟脯氨酸（U-Hyp）与肌苷（U-Cr）。结果：补肾活血液高剂量组的GBMD及FBS均高于27月龄增龄对照组和补肾活血液低剂量组，但低于24月龄本底对照组；高剂量组的BGP明显高于增龄对照组（P<0.05），U-Hyp/Cr明显低于增龄对照组（P<0.05），都与本底对照组相接近；低剂量组的BGP低于本底对照组（P<0.05），U-Hyp/Cr明显高于本底对照组（P<0.05），都与增龄对照组相接近。结论：补肾活血液具有剂量依赖性地降低全身BMD的增龄性减少率和股骨抗弯强度的增龄性降低率，而延缓雄性大鼠增龄性骨质疏松的作用。

Effect of Chinese recipe of replenishing kidney and activating blood

AIM: To evaluate efficiency of Chinese recipe of replenishing kidney and activating blood circulation (CRRKABC) to stave senile osteoporosis(OP) of male rats. METHODS: Forty male SD rats at age o12 months were randomly divided into 4 groups: ①24 months background control; ②27 months aging control; ③CRRKABC (low dose) and ④CRRKABC (high dose). General bone mineral density (GBMD), femur bending strength (FBS), serum-BGP and urine-Hyp/Cr were measured. RESULTS: GBMD and FBS in CRRKABC (H) group were significantly higher than those in 27 months aging control group, but lower than 24 months background control group (P<0.05); GBMD, FBS in CRRKABC groups were slightly higher than those in nylestriol's group (P>0.05). Furthermore, the level of serum-BGP in CRRKABC(H) group was higher and level of U-Hyp/Cr was lower than 27 months aging control group (P<0.05) but very close to that of 24 months background control group. The level of serum-BGP in CRRKABC (L) group was lower and level of U-Hyp/Cr was higher than 24 months background control group (P<0.05) but very close to that of 27 months background control group. CONCLUSION: CRRKABC could control osteoporosis in male rats induced by aging, in a dosedependant manner.